绿原酸抑制HepG_2细胞增殖的体外研究  

Vitro study of chlorogenic acid inhibiting proliferation of HepG_2 cells

作　　者：闫媛[1] 李杰[2] 侯妮[3] 董蕾[4] YAN Yuan LI Jie HOU Ni DONG Lei(Department of Gastroenterology, Xi ＇ an Central Hospital, Xi ＇ an 710003, Chi- net Department of General Surgery, Second Affiliated Hospital of Xi ＇ an Jiaotong University Department of Genetics and Molecular Biolo- gy, Medicine School of Xi＇ an Jiaotong University Department of Gastroenterology, Second Affiliated Hospital of Xi＇ an Jiaotong Universi- ty)

机构地区：[1]西安市中心医院消化科,西安710003 [2]西安交通大学第二附属医院普外科 [3]西安交通大学医学院遗传学与分子生物学研究室 [4]西安交通大学第二附属医院消化科

出　　处：《山西医科大学学报》2017年第6期515-518,共4页Journal of Shanxi Medical University

摘　　要：目的研究不同浓度绿原酸对肝癌细胞的抑制作用,以及对肝癌细胞MAPK/ERK信号通路的影响。方法分别应用50,125,250,500,1 000μmol/L绿原酸与肝癌细胞株HepG_2细胞共孵育24 h,Trypan blue染色并进行HepG_2细胞计数,Western blotting测定HepG_2细胞MAPK/ERK信号通路的ERK1/2磷酸化,CM-H_2DCFDA荧光探针测定HepG_2细胞内ROS浓度,观察绿原酸对HepG_2细胞增殖的抑制作用。结果与0μmol/L组比较,500,1 000μmol/L组HepG_2细胞的增殖分别降低了45.5和81.7个百分点;绿原酸抑制HepG_2细胞MAPK/ERK信号通路的ERK1/2磷酸化(P<0.01);绿原酸促进HepG_2细胞内ROS浓度升高。结论绿原酸通过抑制MAPK/ERK信号通路的活化遏制肝癌细胞增殖。Objective To investigate the effect of chlorogenic acid （CGA） on hepatocarcinoma cells and MAPK/ERK pathway. Methods HepG2 cells were co-incubated with 50,125,250,500,1 000 μmol/L CGA for 24 h respectively. HepG2 cells were counted after Trypan blue staining. ERK1/2 phosphorylation of MAPK/ERK pathway was detected by Western blotting. Intracellular concentra- tion of reactive oxygen species（ROS） was detected using fluorescent probes after treatment with CGA. Results Compared with blank group,the proliferation of HepG2 ceils increased by 45. 5 and 81.7 percentages in 500, 1000 μmol/L CGA group. The 500, 1 000 μmol/L CGA inhibited ERK1/2 phosphorylation（ P 〈 0. 01 ）. Fluorescent probes results showed that CGA promoted ROS regeneration in HepG2 cells. Conclusion CGA may inhibit the proliferation of hepatoearcinoma cells through preventing the activation of MAPK/ ERK pathway.

关 键 词：绿原酸 MAPK/ERK信号通路 HEPG2细胞 

分 类 号：R735.7[医药卫生—肿瘤]