COX-2基因在紫杉醇/卡铂致卵巢癌细胞凋亡中的作用及机制研究  

Role of cyclooxygenase-2 gene in the apoptosis of ovarian cancer cell induced by paclitaxel/carboplatin

作　　者：诸葛飞[1] 童华[2] 高玲娟[2] 王一荃[3] 王祝鸣[4] 

机构地区：[1]南京医科大学附属南京明基医院妇产科,江苏省南京市210019 [2]南京医科大学附属妇产医院,江苏省南京市210004 [3]上海中医药大学附属龙华医院,上海市200032 [4]南京医科大学卫生部抗体技术重点实验室,江苏省南京市210029

出　　处：《实用老年医学》2017年第6期517-520,共4页Practical Geriatrics

基　　金：南京市科技发展项目(201402023)

摘　　要：目的探讨环氧合酶-2(COX-2)在紫杉醇/卡铂致卵巢癌细胞凋亡中的作用及机制。方法以卵巢癌CAOV-3细胞系,卵巢癌组织及其相应的癌旁正常组织为材料,采用实时荧光定量PCR和免疫印迹法分别检测COX-2基因mRNA和蛋白质表达;流式细胞术检测CAOV-3细胞的凋亡情况,同时采用透射电镜观察细胞的超微结构变化。结果 COX-2基因在卵巢癌组织中的表达明显高于其对应的癌旁正常组织(P<0.01);与紫杉醇/卡铂组比较,转染紫杉醇/卡铂+p CMV-COX-2组卵巢癌细胞凋亡率明显减少(P<0.01),且细胞超微结构未发生显著改变。结论 COX-2基因在紫杉醇/卡铂治疗卵巢癌中发挥重要作用,其有望成为卵巢癌治疗的分子新靶标。Objective To investigate the role of cyclooxygenase-2 （COX-2） in the apoptosis of ovarian cancer cells induced by paclitaxel/carboplatin. Methods In ovarian cancer cell line CAOV-3, ovarian cancer tissue, and the corresponding adjacent normal tissue, the expression of COX-2 mRNA gene and protein was explored by Real-time PCR and Western blot, meanwhile, CAOV-3 apoptosis was detected by flow cytometry. Its uhra-microstructure changes were observed by transmission electron microscope. Results The expression of COX-2 mRNA gene was significantly higher in ovarian cancer tissue than that in adjacent normal tissue （ P〈 0. 01 ）. Compared with paclitaxel/carboplatin group, the apoptosis rate of ovarian cancer cells was obviously reduced（ P〈 0.01 ）. And the morphological changes of apoptosis were not significant in paclitaxel/carboplatin ＋pCMV-COX-2 group. Conclusions Cox-2 plays an important role in ovarian cancer cell apoptosis induced by paclitaxel/carboplatin, and may be a new therapy molecular target.

关 键 词：卵巢癌 紫杉醇/卡铂 COX-2 

分 类 号：R737.31[医药卫生—肿瘤]