前列腺癌细胞中特异性标志物lncRNAPCA3的异常表达及其与PRUNE2 mRNA间的相互调控  

Abnormal expression of prostate cancer-specific marker lncRNA PCA3 in prostate cancer cells and its mechanism of mutual regulation with PRUNE2 mRNA

作　　者：周武[1] 季丽丽[1] 张美娟[1] 屠鸿翔[1] 周蓓蓓[1] 

机构地区：[1]温州医科大学附属第一医院,浙江温州325000

出　　处：《中国卫生检验杂志》2017年第11期1595-1597,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省卫生厅科学基金(2015KYB243);国家自然科学青年基金(81202026);温州市科技局(Y20160505)

摘　　要：目的探讨前列腺癌细胞中特异性标志物lncRNA PCA3的异常表达与前列腺癌细胞的异常增殖的关系,以及lncRNA PCA3与邻近的PRUNE2 mRNA间的关系。方法以前列腺癌标准细胞株LNCaP细胞为研究对象,针对lncRNA PCA3和PRUNE2 mRNA分别设计特异性shRNA序列,构建不同shRNA慢病毒转染LNCaP细胞。RT-PCR法检测前列腺癌细胞感染lncRNA PCA3慢病毒后的增殖情况,以及lncRNA PCA3和PRUNE2 mRNA的表达改变。结果慢病毒抑制lncRNA PCA3的表达后,能够检测到LNCaP细胞生长的减慢;相对于阴性对照组,在lncRNA PCA3受到抑制后,PRUNE2 mRNA的表达增加(P<0.05)。反之,当PRUNE2 mRNA的表达受到抑制,则lncRNA PCA3的表达增加(P<0.05)。结论 lncRNA PCA3是影响LNCaP细胞增殖的一个重要因素,其互补链上反向表达的PRUNE2基因是一个抑制lncRNA PCA3表达的抑癌基因,可能是一个未知的有意义的治疗靶点。Objective To investigate the relationship between the aberrant expression of lncRNA PCA3 and the abnormal prolif- eration of prostate cancer cells, and the relationship between lncRNA PCA3 and adjacent PRUNE2 mRNA. Methods Prostate cancer cell LNCaP cells were collected as the subjects. Specific shRNA sequences were designed for IncRNA PCA3 and PRUNE2 mRNA to construct different shRNA lentiviruses, LNCaP cells were transfected with different shRNA lentivirus vec- tors. The proliferation of prostate cancer cells and the expression of lncRNA PCA3 and PRUNE2 mRNA after lentivirus infection was observed by RT - PCR. Results Lentivirus inhibited the expression of lncRNA PCA3, and the slowing down of LNCaP cell can be observed; compared with the negative control group, the expression of PRUNE2 mRNA increased after lncRNA PCA3 was inhibited（ P 〈 0.05 ）. Conversely, when the expression of PRUNE2 mRNA was inhibited, the expression of lncRNA PCA3 increased（ P 〈 0.05 ）. Conclusion LncRNA PCA3 is an important factor which affect the proliferation of LNCaP cells. The reverse - expressed PRUNE2 gene is an anti - oncogene that inhibits the expression of lncRNA PCA3, which may be an un- known and meaningful therapeutic target for prostate cancer.

关 键 词：长链非编码RNA 前列腺癌 基因调控 慢病毒 

分 类 号：R737.25[医药卫生—肿瘤]