TGFβ1-Smad3信号通路参与胃癌条件培养基诱导的网膜脂肪干细胞向癌相关成纤维细胞分化  

TGFβ1 -Smad3 Signal Pathway Participates in the Differentiation of Omental -adipose Stromal Cells towards Carcinoma- associated Fibroblasts Induced by Gastric Cancer

作　　者：张倩[1] 吴晗[1] 龚俊[1] 彭开文[1] 姬忠贺[2] 李雁[2] Zhang Qian Wu Han Gong Jun et al(Department of Oncology,Zhongnan Hospital of Wuhan University,Wuhan 430071 ,China)

机构地区：[1]武汉大学中南医院肿瘤科/肿瘤生物学行为湖北省重点实验室/湖北省肿瘤医学临床研究中心,湖北武汉430071 [2]首都医科大学附属北京世纪坛医院腹膜肿瘤外科,北京100038

出　　处：《医学新知》2017年第3期217-220,F0003,共5页New Medicine

基　　金：2013年湖北省医学领军人才培养工程项目（鄂卫生计生发[2013]4号）;教育部博士点基金（编号：20120141110042）

摘　　要：目的研究TGFN—Smad3信号通路在胃癌条件培养基（CM）诱导网膜脂肪干细胞（O—ASCs）分化为癌相关成纤维细胞（CAFs）中的作用。方法通过诱导分化成骨、成脂及流式细胞鉴定O—ASCs，将O—ASCs分为对照组、SGC7901-CM试验组、SGC7901-CM＋SB431542处理组，12h后收集细胞。通过RT—PCR和Western—blot检测各组细胞CAFs标志物α—SMA、FSP-1的表达水平。通过Western—blot检测Smad信号通路Smad3、P—Smad3的表达水平。结果鉴定原代培养细胞为O—ASCs。与对照组相比，SGC7901-CM组CAFs特异性标志物仪-SMA、FSP-1及Smad信号通路蛋白P—Smad3均增加明显（P〈0．05），Smad3表达未见明显变化（P〉0．05）。与SGC7901-CM组相比，SGC7901-CM＋SB431542组仪-SMA，FSP-1及P—Smad3蛋白表达均受到明显抑制（P〈0．05），Smad3表达未见明显变化（P〉0．05）。结论胃癌条件培养基具有诱导O—ASCs向CAFs分化的作用，TGFβ1-Smad3信号通路在该过程中发挥了重要作用。Objective To investigate effects of TGFβ1 - Smad3 signal pathway on the differentiation of omental - adipose stromal ceils（ O- ASCs）towards carcinoma- associated fibroblasts （CAFs）induced by gastric cancer condi- tioned medium（CM）. Methods O - ASCs were identified by examining their ability to differentiate into osteogenic and adipogenic lineages and flow cytometry. O - ASCs were divided into control group, SGC7901 - CM group and SGC7901 -CM ＋ SB431542 group. Cells were collected after 12 hours. RT- PCR and western- blot were utilized to evaluate the expression of α - SMA and FSP - 1. Western-blot was utilized to evaluate the expression of Smad3 and p - Smad3. Results Primary ceils were verified as O - ASCs. The expression of α - SMA, FSP - 1 and p - Smad3 in- creased（ P 〈 0.05 ） and the expression of Smad3 didnt change significantly （ P 〉 0.05 ） in SGC7901 - CM group than those in control group. The expression of α - SMA, FSP - 1 and p - Smad3 decreased （ P 〈 0.05 ） and the expression of Smad3 didnt change significantly（ P 〉0.05 ）in SGC7901 -CM ＋ SB431542 group than those in SGC7901 -CM group （ P 〈 0.05）. Conclusion O - ASCs can be induced to CAFs exposed to gastric cancer conditioned medium. TGFI31 - Smad3 sienal uathwav olavs an imoortant role in the differentiation of O -ASCs towards CAFs.

关 键 词：胃癌 癌相关成纤维细胞 网膜脂肪干细胞 TGFβ1-Smad3信号通路 

分 类 号：R735.2[医药卫生—肿瘤]