毛细管电泳法测定(S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基)乙酸甲酯中R异构体  

Determination of R isomer in (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl) acetate by capillary electrophoresis

作　　者：潘文燕[1] 闫玉刚[1] 侯准[1] 杨新颖[1] 方浩[1] 

机构地区：[1]山东大学药学院,山东济南250012

出　　处：《现代药物与临床》2017年第5期776-779,共4页Drugs & Clinic

基　　金：山东省自然科学基金杰出青年基金资助项目(JQ201319);山东大学自主创新基金资助项目(2016JC018)

摘　　要：目的建立毛细管电泳法测定(S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基)乙酸甲酯中R异构体的方法。方法采用毛细管电泳法。以磺酸-β-环糊精(S-β-CD)为选择剂;25 mmol/L硼酸盐缓冲液(pH 8.9,含S-β-CD 1.8%)为运行缓冲液;运行电压为25 kV;柱温为15℃;检测波长:214 nm;3.4 kPa压力进样10 s。结果 (S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基)乙酸甲酯与R异构体的分离度为2.9。R异构体在2~30μg/m L与峰面积线性关系良好(r=0.999 5),平均回收率为96.6%,RSD值为4.9%(n=6)。结论建立的毛细管电泳法操作简便,结果准确可靠,可用于(S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基)乙酸甲酯中R异构体的控制。Objective To establish a capillary electrophoresis method for the determination of R-optical isomer in（S）-methyl-2-（6-hydroxy-2,3-dihydrobenzofuran-3-yl）acetate. Methods Capillary electrophoresis method was adopted. Sulfate-β-cyclodextrin（S-β-CD） was used as selector. The background buffer was 25 mmol/L borate buffer solution（pH 8.9, containing 1.8% S-β-CD）. The separation was performed at 15 ℃ with the voltage of 25 k V and the wavelength was 214 nm. Pressure was 3.4 k Pa and injected for 10 s. Results（S）-methyl-2-（6-hydroxy-2,3-dihydrobenzofuran-3-yl）acetate and its R-enantiomer were basely resolved with resolution of 2.9. The calibration curve of R-enantiomer was linear in the concentration range of 2 — 30 μg/mL（r = 0.999 5）. The average recovery was 96.6% with RSD 4.9%（n = 6）. Conclusion The method is convenient, rapid, accurate, and can be used for the determination of（R）-methyl-2-（6-hydroxy-2,3-dihydrobenzofuran-3-yl）acetate in（S）-methyl-2-（6-hydroxy-2,3-dihydrobenzofuran-3-yl） acetate.

关 键 词：(S)-2-(6-羟基-2 3-二氢苯并呋喃-3-基)乙酸甲酯 R异构体 毛细管电泳 

分 类 号：R927.2[医药卫生—药学]