Reproductive toxicity assessment of benzo[a]pyrene in the marine polychaete Perinereis nuntia  

作　　者：吴清洋 王树启 陈晓鹏 李平 

机构地区：[1]Guangdong Provincial Key Laboratory of Marine Biology,Shantou University [2]Administration of Ocean and Fisheries of Shantou

出　　处：《Chinese Journal of Oceanology and Limnology》2017年第4期867-873,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the Guangdong High-Level University Project"Green Technologies for Marine Industries"(No.130-33106703);Guangdong Natural Science Foundation(2016A030313065);the Outstanding Young Teachers of Guangdong Colleges and Universities(No.YQ2015074)

摘　　要：Benzo[a]pyrene （B[a]P） is an increasingly present marine environmental pollutant, yet our understanding of the long-term consequences of reproductive toxicity in marine benthic polychaetes remains limited. To test the reproductive toxicity of B[a]P on polychaetes, Perinereis nuntia was exposed to B[a]P-contaminated artificial seawater and sexual maturation, the sex ratio, number of eggs spawned, fertilization and hatching rated, as well as vitellogenin （VTG） mRNA expression levels were analyzed. A low concentration of B[a]P （2.5 gg/L） had no effects on the rate of sexual maturation, spawning, or fertilization but significantly increased the sex ratio （female： male） from 1.6±0.15：1 to 2.3±0.18：1, inhibited hatching rate by 27%, and significantly increased VTG mRNA expression level by 3.7-fold following a 60-day exposure, compared with those in the solvent controls. A higher concentration of B[a]P （25 μg/L） caused more serious effects; sexual maturation, fertilization success, and hatching decreased by 31%, 17% and 46%, respectively, and the sex ratio （female： male） and VTG mRNA gene expression level increased by 54% and 7.1-fold, respectively. These results demonstrate that sublethal concentrations of B[a]P negatively affect reproductive performance of the sandworm P. nuntia.Benzo[a]pyrene(B[a]P) is an increasingly present marine environmental pollutant,yet our understanding of the long-term consequences of reproductive toxicity in marine benthic polychaetes remains limited.To test the reproductive toxicity of B[a]P on polychaetes,Perinereis nuntia was exposed to B[a]P-contaminated artificial seawater and sexual maturation,the sex ratio,number of eggs spawned,fertilization and hatching rated,as well as vitellogenin(VTG) mRNA expression levels were analyzed.A low concentration of B[a]P(2.5μg/L) had no effects on the rate of sexual maturation,spawning,or fertilization but significantly increased the sex ratio(female:male) from 1.6±0.15:1 to 2.3±0.18:1,inhibited hatching rate by 27%,and significantly increased VTG mRNA expression level by 3.7-fold following a60-day exposure,compared with those in the solvent controls.A higher concentration of B[a]P(25μg/L)caused more serious effects;sexual maturation,fertilization success,and hatching decreased by 31%,17% and 46%,respectively,and the sex ratio(female:male) and VTG mRNA gene expression level increased by54% and 7.1-fold,respectively.These results demonstrate that sublethal concentrations of B[a]P negatively affect reproductive performance of the sandworm P.nuntia.

关 键 词：Perinereis nuntia BENZO[A]PYRENE reproductive biology VITELLOGENIN 

分 类 号：X171.5[环境科学与工程—环境科学]