Homology-mediated end joining-based targeted integration using CRISPR/Cas9  

作　　者：Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang[1] Shi, Linyu[1] Yang, Hui[1] 

机构地区：[1]Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Key Lab Primate Neurobiol,Inst Neurosci,State Key, Shanghai 200031, Peoples R China [2]Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China [3]ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China [4]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China [5]Shanghai Univ, Shanghai 200444, Peoples R China [6]Natl Inst Biol Sci, Beijing 102206, Peoples R China

出　　处：《Cell Research》2017年第6期801-814,共14页细胞研究（英文版）

摘　　要：Targeted integration of transgenes can be achieved by strategies based on homologous recombination （HR）, mi- crohomology-mediated end joining （MMEJ） or non-homologous end joining （NHEJ）. The more generally used HR is inefficient for achieving gene integration in animal embryos and tissues, because it occurs only during cell division, although MMEJ and NHEJ can elevate the efficiency in some systems. Here we devise a homology-mediated end joining （HMEJ）-based strategy, using CRISPR/Cas9-mediated cleavage of both transgene donor vector that contains guide RNA target sites and -800 bp of homology arms, and the targeted genome. We found no significant improve- ment of the targeting efficiency by the HMEJ-based method in either mouse embryonic stem cells or the neuroblas- toma cell line, N2a, compared to the HR-based method. However, the HMEJ-based method yielded a higher knock- in efficiency in HEK293T cells, primary astrocytes and neurons. More importantly, this approach achieved transgene integration in mouse and monkey embryos, as well as in hepatocytes and neurons in vivo, with an efficiency much greater than HR-, NHEJ- and MMEJ-based strategies. Thus, the HMEJ-based strategy may be useful for a variety of applications, including gene editing to generate animal models and for targeted gene therapies.

关 键 词：homology-mediated end joining CRISPR/Cas9 monkey embryos NEURONS KNOCK-IN 

分 类 号：Q55[生物学—生物化学] S511.03[农业科学—作物学]