云南高校图书馆联盟文献共享服务平台- KNOCK-IN

KNOCK-IN: 作品数：25被引量：104H指数：5; 导出分析报告; 相关领域：生物学医药卫生更多>>; 相关作者：王红胡静姜元于翠娟吴智群更多>>; 相关机构：吉林大学陕西师范大学广西大学国立中兴大学更多>>; 相关期刊：《Advances in Bioscience and Biotechnology》《Bone Research》《Journal of Animal Science and Biotechnology》《Science China(Life Sciences)》更多>>; 相关基金：国家自然科学基金中国博士后科学基金广东省自然科学基金北京市自然科学基金更多>>

Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth,Plutella xylostella: 《Journal of Integrative Agriculture》2024年第9期3089-3103,共15页Shanyu Li Guifang Lin Haoqi Wen Haiyan Lu Anyuan Yin Chanqin Zheng Feifei Li Qingxuan Qiao Lu Jiao Ling Lin Yi Yan Xiujuan Xiang Huang Liao Huiting Feng Yussuf Mohamed Salum Minsheng You Wei Chen Weiyi He; supported by the National Natural Science Foundation of China(32172503 and 32260721);the Natural Science Foundation of Fujian Province,China(2023J01069);the State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,China(SKL2022001);the Innovation Fund of Fujan Agriculture and Forestry University,China(KFB23014A);the Undergraduate Training Program for Innovation and Entrepreneurship of Fujian Province,China(S202210389101).; Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome editing.In the current study,we showed that the mutation of single-allele Px...; 关键词：cruciferous specialist fl(2)d CRISPR/Cas9 KNOCK-IN sex chromosome

Efficient scar-free knock-ins of several kilobases in plants by engineered CRISPR-Cas endonucleases: 《Molecular Plant》2024年第5期824-837,共14页Tom Schreiber Anja Prange Petra Schafer Thomas Iwen Ramona Grutzner Sylvestre Marillonnet Aurelie Lepage Marie Javelle Wyatt Paul Alain Tissier; funded by grant no.031B0548 in the frame of the program"Crop plants of the future"from the Bundesministerium fur Bildung und Forschung to A.T.;funded by the Investissement d’Avenir program of the French National Agency of Research for the project GENIUS(ANR-11-BTBR-0001_GENIUS).; In plants and mammals,non-homologous end-joining is the dominant pathway to repair DNA doublestrand breaks,making it challenging to generate knock-in events.In this study,we identified two groups of exonucleases from ...; 关键词：homology-directed repair knockin gene replacement CRISPR-Cas 50 exonuclease PLANTS

Superior Fidelity and Distinct Editing Outcomes of SaCas9 Compared with SpCas9 in Genome Editing被引量：1: 《Genomics, Proteomics & Bioinformatics》2023年第6期1206-1220,共15页Zhi-Xue Yang Ya-Wen Fu Juan-Juan Zhao Feng Zhang Si-Ang Li Mei Zhao Wei Wen Lei Zhang Tao Cheng Jian-Ping Zhang Xiao-Bing Zhang; supported by the National Natural Science Foundation of China(Grant Nos.82070115,81770198,81870149,81970121,and 8142100);the National Key R&D Program of China(Grant Nos.2019YFA0110803,2019YFA0110802,2019YFA0110204,and 2016YFA0100600);the Tianjin Municipal Science and Technology Commission Grant(Grant No.19JCZDJC33000);the CAMS Innovation Fund for Medical Sciences(Grant Nos.2017-I2M-2-001,2017-I2M-B&R-04,and 2019-I2M-1-006).; A series of clustered regularly interspaced short palindromic repeats(CRISPR)-CRISPR associated protein 9(Cas9)systems have been engineered for genome editing.The most widely used Cas9 is SpCas9 from Streptococcus pyo...; 关键词：SpCas9 SaCas9 Spacer length Indel pattern Knock-in efficiency Off-target

A mutation in the ZNF687 gene that is responsible for the severe form of Paget's disease of bone causes severely altered bone remodeling and promotes hepatocellular carcinoma onset in a knock-in mouse model被引量：3: 《Bone Research》2023年第1期201-214,共14页Sharon Russo Federica Scotto di Carlo Antonio Maurizi Giorgio Fortunato Anna Teti Danilo Licastro Carmine Settembre Tommaso Mello Fernando Gianfrancesco; Italian Association for Cancer Research(AIRC)under IG 2020-ID.25110 project;POR Campania FESR 2014/2020(Project SATIN);Progetto MISE(F/050011/02/X32)–P.I.Fernando Gianfrancesco;funding from the European Calcified Tissue Society(ECTS)and the Italian Society for Osteoporosis,Mineral Metabolism and Skeletal Diseases(SIOMMMS)。; Paget’s disease(PDB)is a late-onset bone remodeling disorder with a broad spectrum of symptoms and complications.One of the most aggressive forms is caused by the P937R mutation in the ZNF687 gene.Although the geneti...; 关键词：RAW264.7 PAGET REMODELING

The collagen type Ⅰ alpha 1 chain gene is an alternative safe harbor locus in the porcine genome被引量：1: 《Journal of Integrative Agriculture》2023年第1期202-213,共12页XIANG Guang-ming ZHANG Xiu-ling XU Chang-jiang FAN Zi-yao XU Kui WANG Nan WANG Yue CHE Jing-jing XU Song-song MU Yu-lian LI Kui LIU Zhi-guo; supported by the Major Scientific Research Tasks for Scientific and Technological Innovation Projects of the Chinese Academy of Agricultural Sciences(CAAS-ZDRW202006);the National Transgenic Breeding Project(2018ZX08010-10B);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP-IAS05).; Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent o...; 关键词：COL1A1 gene safe harbor KNOCK-IN CRISPR/Cas9 PIG

Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression被引量：5: 《Science China(Life Sciences)》2022年第11期2269-2286,共18页Qin Jin Xiaoyu Yang Shixue Gou Xiaoyi Liu Zhenpeng Zhuang Yanhui Liang Hui Shi Jiayuan Huang Han Wu Yu Zhao Zhen Ouyang Quanjun Zhang Zhaoming Liu Fangbing Chen Weikai Ge Jingke Xie Nan Li Chengdan Lai Xiaozhu Zhao Jiaowei Wang Meng Lian Lei Li Longquan Quan Yinghua Ye Liangxue Lai Kepin Wang; the National Key Research and Development Program of China(2017YFA0105103,2021YFA0805903);the National Natural Science Foundation of China(81941004,32170542);2020 Research Program of Sanya Yazhou Bay Science and Technology City(202002011);Major Science and Technology Projects of Hainan Province(ZDKJ2021030);Key Research&Development Program of Hainan Province(ZDYF2021SHFZ052);Youth Innovation Promotion Association of the Chinese Academy of Sciences(2019347);Young Elite Scientist Sponsorship Program by CAST(YESS20200024);Biological Resources Progaramme,Chinese Academy of Sciences(KFJBRP-017-57);Key Research&Development Program of Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory)(2018GZR110104004);China Postdoctoral Science Foundation(2020M682943);Science and Technology Planning Project of Guangdong Province,China(2019A030317010,2020B1212060052,2021B1212040016,2021A1515011110);Science and Technology Program of Guangzhou,China(202007030003);Research Unit of Generation of Large Animal Disease Models,Chinese Academy of Medical Sciences(2019-I2M-5-025)。; Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological process.Binary Tet-On system has been widely employed to regulate transgenic expression by doxycycline.Previous...; 关键词：double knock-in Tet-On system phiC31-mediated cassette exchange pig model hKRASG12D-related tumorigenesis

Characterization of two novel knock-in mouse models of syndromic retinal ciliopathy carrying hypomorphic Sdccag8 mutations: 《Zoological Research》2022年第3期442-456,共15页Zhi-Lin Ren Hou-Bin Zhang Lin Li Zheng-Lin Yang Li Jiang; supported by the Natural Science Foundation of China(81670893,82121003);Science and Technology Department of Sichuan Province(2021JDZH0031);Chinese Academy of Medical Sciences(2019-I2M-5-032)。; Mutations in serologically defined colon cancer autoantigen protein 8(SDCCAG8)were first identified in retinal ciliopathy families a decade ago with unknown function.To investigate the pathogenesis of SDCCAG8-associat...; 关键词：SDCCAG8 Primary cilia Retinal ciliopathy Bardet-Biedl syndrome(BBS) Senior-L?ken syndrome(SLS) Nephronophthisis(NPHP) POLYDACTYLY

LRRK2 mutant knock-in mouse models: therapeutic relevance in Parkinson’s disease被引量：1: 《Translational Neurodegeneration》2022年第1期790-808,共19页Eunice Eun Seo Chang Philip Wing-Lok Ho Hui-Fang Liu Shirley Yin-Yu Pang Chi-Ting Leung Yasine Malki Zoe Yuen-Kiu Choi David Boyer Ramsden Shu-Leong Ho; Tai Hung Fai Charitable Foundation-Edwin S H Leong Research Programme for Parkinson’s Disease;The Henry G.Leong Endowed Professorship in Neurology;The Donation Fund for Neurology Research;Health and Medical Research Fund(HMRF),Food and Health Bureau,Hong Kong S.A.R.; Mutations in the leucine-rich repeat kinase 2 gene (LRRK2) are one of the most frequent genetic causes of both familial and sporadic Parkinson’s disease (PD). Mounting evidence has demonstrated pathological similarit...; 关键词：Parkinson’s disease LRRK2 Knock-in mouse model NEUROTRANSMISSION Motor dysfunction Autophagy LYSOSOME Mitochondrial dysfunction SYNUCLEINOPATHY Hyperkinase activity LRRK2 inhibitor

An App knock-in rat model for Alzheimer’s disease exhibiting Aβand tau pathologies,neuronal death and cognitive impairments被引量：14: 《Cell Research》2022年第2期157-175,共19页Keliang Pang Richeng Jiang Wei Zhang Zhengyi Yang Lin-Lin Li Makoto Shimozawa Simone Tambaro Johanna Mayer Baogui Zhang Man Li Jiesi Wang Hang Liu Ailing Yang Xi Chen Jiazheng Liu Bengt Winblad Hua Han Tianzi Jiang Weiwen Wang Per Nilsson Wei Guo Bai Lu; This work was supported by the National Key Research and Development Program of China(2017YFE0126500);the National Natural Science Foundation of China(81861138013,81501105,31730034);Beijing Advanced Innovation Center for Human Brain Protection,Shenzhen Science Technology and Innovation Commission(JCYJ20170411152419928 and JCYJ20180508152240368);Beijing Municipal Science&Technology Commission(Z151100003915118)to B.L.,W.G.;K.P.Strategic Priority Research Program of Chinese Academy of Science(NO.XDB32030200)to HH.We acknowledge funding from Hållstens forskningsstiftelse,Swedish Research Council,Swedish Brain foundation,Swedish Alzheimer foundation,Margaretha af Ugglas Foundation,Sonja Leikrans donation,China Scholarship Council and H2020-MSCA-ITN-2019#860035(R.J.,M.S.,S.T.,J.W.,B.W.and P.N.).We thank Takaomi Saido and Takashi Saito at RIKEN Center for Brain Science,Japan for providing the App knock-in mice to Department of Neurobiology,Care Sciences and Society,Division of Neurogeriatrics,Karolinska Institutet.We thank Dr.P.Davies(Albert Einstein College of Medicine,Bronx,NY)for antibodies Alz-50,and MC-1,Dr.Jianzhi Wang(Tongji Medical College,Huazhong University&Technology,Wuhan,Hubei)for APP/PS1 mice frozen brain sections,Dr.Chuanzhou Li and Dr.Hao Li(Tongji Medical College,Huazhong University&Technology,Wuhan,Hubei)for P301 mice frozen brain sections,Dr.Mengchao Cui(College of Chemistry,Beijing Normal University,Beijing)for P301 mice paraffin brain sections,and Xaomin Wang(Capital Medical University,Beijing)for 5×FAD mice frozen brain sections.We thank the Imaging Core Facility,the Technology Center for Protein Sciences(Tsinghua University)for assistance on confocal imaging and Imaris software data analysis.We also thank Dr.Yichang Jia(School of Medicine,Tsinghua University,Beijing)for the discussion of gene editing strategy and Dr.Guhan Nagappan for manuscript discussion and editing.CRISPR/Cas9 manipulation was done by a CRO company(Beijing Biocytogen Co.,Ltd).; A major obstacle in Alzheimer’s disease(AD)research is the lack of predictive and translatable animal models that reflect disease progression and drug efficacy.Transgenic mice overexpressing amyloid precursor protein...; 关键词：TAU AΒ ALZHEIMER

Essential role of Msx1 in regulating anterior-posterior patterning of the secondary palate in mice被引量：2: 《Journal of Genetics and Genomics》2022年第1期63-73,共11页Shicheng Zhu Hanjing Song Liangjun Zhong Suman Huo Yukun Fang Wanxin Zhao Xueqin Yang Zhong-Min Dai Rui He Mengsheng Qiu Zunyi Zhang Xiao-Jing Zhu; supported by grants from the National Natural Scientific Foundation of China (81771028 and 317771593);Medical Health Science and Technology Project of Zhejiang (2021KY891);Medical Health Science and Technology Major Project of Hangzhou (Z20200046)。; Development of the secondary palate displays molecular heterogeneity along the anterior-posterior axis;however, the underlying molecular mechanism remains largely unknown. MSX1 is an anteriorly expressed transcription...; 关键词：Cleft palate Conditional knock-in Mouse model MSX1 Organ development

高级检索 检索式检索

时间限定

期刊范围

学科限定全选

高级检索 检索式检索

时间限定

期刊范围

学科限定全选

KNOCK-IN

检索结果分析

下载全文

高级检索检索式检索

时间限定

期刊范围

学科限定全选

高级检索 检索式检索

时间限定

期刊范围

学科限定全选

KNOCK-IN

检索结果分析

下载全文

用户登录

高级检索检索式检索