盐酸罗哌卡因对人乳腺癌细胞运动作用的研究  

Study on Effect of Ropivacaine on Migration of Breast Cancer Cells

作　　者：唐努尔.艾尔肯 阿孜古.祖农 

机构地区：[1]新疆喀什地区第一人民医院麻醉科,新疆喀什844000 [2]新疆喀什地区肺科医院检验科,新疆喀什844000

出　　处：《现代检验医学杂志》2017年第3期128-130,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨不同剂量的盐酸罗哌卡因对乳腺癌MDA-MB-231细胞生长和运动行为学的影响。方法乳腺癌MDA-MB-231细胞常规培养后加入不同剂量盐酸罗哌卡因。分组为低剂量组150μg/ml(A组),中剂量组250μg/ml(B组)和高剂量组450μg/ml(C组),设空白对照组(D组)。MTT法检测细胞生长情况,同时观察细胞划痕和趋化运动能力变化。结果低剂量盐酸罗哌卡因组乳腺癌细胞生长不受影响,而中剂量和高剂量组乳腺癌细胞生长受到较大的抑制,与对照组相比差异有统计学意义(t=8.845~11.654,P<0.05)。低剂量盐酸罗哌卡因组乳腺癌细胞的迁移和趋化运动能力与对照组相比差异无统计学意义(t=1.025~1.124,P>0.05)。中剂量组和高剂量组乳腺癌细胞的迁移和趋化运动能力比对照组明显减低,组间差异有统计学意义(t=6.054~12.412,t=8.654~10.214,均P<0.05)。结论盐酸罗哌卡因能抑制乳腺癌细胞生长和运动,具有剂量依赖性。Objective To observe the different behavior of migration in breast cancer cells, when exposure to ropivacaine of different concentrations. Methods The breast cancer cells MDA-MB-231 were inoculated into culture medium. They were divided into four groups ： low-dose group （ 150 μg/ml, A group）, medium-dose group （250 μg/ml, B group）, high-dose group （450 μg/ml,C group） and control group （D group） ,and detected the cell proliferation,abilities of cell scratch and chemotax is. Results MDA-MB-231 cell viability of B and C group was significantly lower than that of D group （t=8. 845-11. 654, P=0.05）. Migration and chemotaxis abilities of B group and C group were decreased than those of D group （t= 6. 054- 12. 412,t=8. 654-10. 214,P〈0.05）,and there was no difference between A group and D group （t= 1. 025-1. 124,P〉 0.05）. Conclusion Ropivacaine could restrain the viability and migration abilities of breast cancer cells depending on its different concentrations.

关 键 词：盐酸罗哌卡因 乳腺癌 迁移 趋化运动 

分 类 号：R737.9[医药卫生—肿瘤] R730.43[医药卫生—临床医学]