14-3-3σ基因表达下调促进肾癌细胞增殖  

Effects of down-regulation of gene expression of 14-3-3σ on proliferation of renal cancer cells

作　　者：仇炜[1] 沈永青[2] 张爱莉[3] 

机构地区：[1]首都医科大学附属北京友谊医院泌尿外科,北京市100050 [2]河北中医学院护理学院 [3]河北医科大学第四医院泌尿外科

出　　处：《河北医药》2017年第14期2085-2089,共5页Hebei Medical Journal

摘　　要：目的检测14-3-3σ在肾癌(renal cell carcinoma,RCC)及相应正常肾组织(normal tissue,NT)中的mRNA及蛋白表达情况,探讨14-3-3σ在RCC中的作用及其作用机制。方法随机选择47例经肾肿瘤根治性手术治疗的肾癌患者,提取肾癌组织及正常肾组织mRNA及蛋白,使用RT-PCR法检测肾癌及正常肾组织中14-3-3σmRNA的表达情况,使用Western blot方法检测14-3-3σ蛋白在肾癌及正常肾组织中的表达水平,统计肾癌组织及正常肾组织中14-3-3σ基因的表达差异。转染肾癌786-O细胞,分别使用质粒过表达或siRNA敲低14-3-3σ基因的表达,使用CCK-8法检测786-O细胞在过表达和敲低14-3-3σ基因后的增殖变化。结果肾癌组织较正常肾组织14-3-3σ基因mRNA表达水平明显降低(P<0.01),且该基因在肾癌组织中的蛋白表达水平也较正常肾组织明显降低(P<0.01)。在786-O细胞内过表达14-3-3σ基因能够明显抑制786-O细胞的增殖(P<0.05),敲低该基因的表达能够明显促进786-O细胞增殖(P<0.01)。结论肾癌组织较正常肾组织14-3-3σ基因的表达水平明显下降,14-3-3σ与肾癌细胞的增殖有关。Objective To investigate the expressions of 14-3-3σ in renal cell carcinoma tissues（ RCC） and in normal renal tissues（ NT）,and to explore the role of 14-3-3σ and its action mechanism in RCC. Methods Forty-seven patient with renal cancer who enderwent radical nephrectomy were enrolled in the study. The RNA and protein were extracted from RCC tissues and normal renal tissues,and the expression levels of 14-3-3σ were detected by RT-PCR and Western blot,respectively. The changes of cell proliferation were detected by CCK-8 assays in 786-O cells after overexpression or knockdown of gene expressions of 14-3-3σ. Results As compared with those in normal renal tissues,the expression levels of 14-3-3σ mRNA and protein in renal cancer tissues were significantly decreased（ P 〈0. 01）,moreover,the overexpression of 14-3-3 σ gene could significantly inhibit the proliferation of 786-O cells（ P〈 0. 05）,however,the knockdown of 14-3-3σ gene expression could increase the cell proliferation（ P 〈0. 01）. Conclusion The expression levels of 14-3-3σ gene are significantly down-regulated in RCC tissues,which may be correlated to the the proliferation of renal cancer cells.

关 键 词：肾肿瘤 14-3-3σ 增殖 RT-PCR 

分 类 号：R734.2[医药卫生—肿瘤]