免疫学检测中抗体固定化方法的研究现状  

A review on antibody immobilization in immunoassays

作　　者：刘爱平[1] 申文浩[1] 王小红[2] 李诚[1] 

机构地区：[1]四川农业大学食品学院,四川雅安625014 [2]华中农业大学食品学院,湖北武汉430070

出　　处：《江苏农业学报》2017年第3期714-720,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31271876);中央高校基本科研业务费专项(2013PY103;52209-814012)

摘　　要：免疫学检测技术,因具有快速、灵敏、成本低等优点,被广泛应用于食品安全、环境监测等领域。抗体是免疫学检测的核心试剂,免疫学检测中常需要对抗体进行固定,高效固定抗体对提高检测灵敏度具有重要意义。本文对抗体固定化各类方法的特点及其在免疫学检测中的应用进行综述,并对抗体定向固定化技术发展趋势进行了展望。Immunological assays are widely applied in food safety and environmental monitoring because they are rapid, sensitive and of low price. Antibody, the core reagent in immunological assays, usually needs to be immobilized to capture antigen. It is of great significance to improve detection sensitivity through effective immobilization of antibody. This review seeks to overview the characteristics and application of each kind of immobilization methods in immunological assays, and the developing trends of oriented-immobilization technique were prospected.

关 键 词：抗体 定向固定 生物素-亲和素 亲和肽配基 DNA引导固定法 

分 类 号：Q599[生物学—生物化学]