防己诺林碱联用紫杉醇对耐药卵巢癌SKOV3/ADM细胞作用机制的初步研究  

Effect of fangchinoline combined with paclitaxel on drug resistant ovarian cancer SKOV3/ADM cells: a preliminary study

作　　者：何丽 林永红 李笑晓[2] 张芸榕[2] 杨俊毅[2] 

机构地区：[1]成都市妇女儿童中心医院妇产科,四川成都610091 [2]四川大学华西药学院临床药学研究中心,四川成都610041

出　　处：《中国计划生育和妇产科》2017年第6期59-62,共4页Chinese Journal of Family Planning & Gynecotokology

基　　金：四川省卫生厅科学研究项目(项目编号:110069)

摘　　要：目的评价防己诺林碱调控紫杉醇对耐药卵巢癌SKOV 3/ADM细胞多药耐药的作用。方法采用MTT法和细胞摄取研究防己诺林碱调控紫杉醇对SKOV 3/ADM细胞多药耐药的作用。结果细胞生长抑制试验表明,紫杉醇对SKOV 3/ADM细胞耐药明显。0.5、1和5μg·mL^(-1)浓度的防己诺林碱分别与紫杉醇联用后,紫杉醇对细胞的半数抑制浓度(IC_(50))降为(0.542±0.117)、(0.924±0.153)和(1.931±0.375)μg·mL^(-1),耐药逆转倍数If分别为16.1、9.4和4.5倍,与单用紫杉醇相比差异有统计学意义(P<0.05)。细胞摄取试验研究表明,联用防己诺林碱浓度达到0.5μg·mL^(-1)及以上时,能够显著增加紫杉醇的细胞摄取(P<0.05)。结论防己诺林碱是潜在的逆转紫杉醇对SKOV 3/ADM细胞多药耐药候选活性化合物。Objective To evaluate muhidrug resistance of SKOV3/ADM cell for paclitaxel regulated by fangchinoline. Methods MTT tests and cell aptaking were adopted to research the activity of fangchinoline in regulating the muhidrug resistance effect of paclitaxel to SKOV3/ADM. Results Cell growth inhibition test showed that paclitaxel was significantly resistant to SKOV3/ADM cells. Half inhibitory concentration （IC50） decreased to （0. 542 ± 0. 117） ,（0. 924 ± 0. 153）and（1. 931 ± 0. 375） μg · mL-1, respectively, after the combined administration of paclitaxel with 0. 5, 1 and 5 μg · mL-1concentrations of fangchinoline. The reversal factor of resistance was 16. 1, 9.4 and g. 5 times, respectively, which was significantly different from that of paclitaxel alone（P 〈 0.05）. Cell uptake studies showed that, Fangehinoline could increase cell uptake pencentage of paclitaxel when the concerntration was higher than 0. 5 μg ·mL- 1 （ P 〈 0.05 ）. Conclusion Fangchinoline is a potential reversal of paclitaxel on SKOV 3 / ADM cells in multidrug resistant candidate active compounds.

关 键 词：防己诺林碱 紫杉醇 卵巢癌 多药耐药 MTT试验 

分 类 号：R730.53[医药卫生—肿瘤]