慢性牙周炎患者血清中TGF-β各亚型的定量分析  

Quantitative Analysis of Serum TGF-βIsoforms in Chronic Periodontitis Patients

作　　者：李爽[1] 孟晓雪[2] 周建业[3] 易根云[1] 黄慧敏[1] 张小凤[1] 李志强[3] 余占海[1] 

机构地区：[1]兰州大学口腔医学院,甘肃兰州730000 [2]兰州大学第一医院,甘肃兰州730000 [3]西北民族大学口腔医学院,甘肃兰州730000

出　　处：《口腔医学研究》2017年第6期618-622,共5页Journal of Oral Science Research

基　　金：国家自然科学基金地区项目(编号:31560159/C0309);西北民族大学中央高校重点培育项目(编号:31920170162);四川大学口腔疾病研究国家重点实验室开放课题(编号:SKLOD20170F07)

摘　　要：目的:检测慢性牙周炎患者血清中TGF-β各亚型(TGF-β1、TGF-β2、TGF-β3)的表达差异,探讨三者在慢性牙周炎中的作用。方法:研究对象分为中度牙周炎组(n=9)、重度牙周炎组(n=18)和健康对照组(n=19),基于Lumninex 200多重分析仪对其血清进行定量分析。结果:对照组血清中TGF-β1浓度(41053.26±8318.65)ng/L及TGF-β3浓度(2454.58±552.73)ng/L含量水平均高于中度牙周炎组分别为(32112.22±7153.87)ng/L,P<0.01;(1991.11±532.22)ng/L,P<0.05)和重度牙周炎组分别为(30652.89±8245.31)ng/L,P<0.01;(1829.17±483.68)ng/L,P<0.01);TGF-β1和TGF-β3分别与CAL、PD、BOP、PLI呈明显负相关;TGF-β1与TGF-β3呈明显正相关。结论:血清中浓度较高的TGF-β1可能对慢性牙周炎的发生发展起一定的抑制作用,而TGF-β3不仅对慢性牙周炎的发生发展起一定的抑制作用而且可能与TGF-β1存在协同作用。Objective：To examine the role of TGF-βisoforms（TGF-β1,TGF-β2and TGF-β3）in chronic periodontitis.Methods：The research subjects were divided into moderate periodontitis group（n=9）,severity periodontitis group（n=18）,and healthy controls（n=19）.Then their serum was detected through Luminex200 based multi-analytic assay.Results：The levels of serum TGF-β1（41053.26±8318.65）ng/L and TGF-β3（2454.58±552.73）ng/L in control group were significantly higher than those in moderate periodontitis group（32112.22±7153.87）ng/L,P〈0.01;（1991.11±532.22）ng/L,P〈0.05,respectively and severity periodontitis group（30652.89±8245.31）ng/L,P〈0.01;（1829.17±483.68）ng/L,P〈0.01.TGF-β1and TGF-β3were negative correlated with CAL,PD,BOP and PLI.There was a strong positive correlation between TGF-β1and TGF-β3.Conclusion：Higher concentration of TGF-β1in serum may have inhibitory effect on the development of chronic periodontitis.TGF-β3also has a similar inhibitory effect and it maybe interact with TGF-β1.

关 键 词：慢性牙周炎 血清 Luminex多重分析 TGF-β亚型 

分 类 号：R781.4[医药卫生—口腔医学]