血红素氧化酶Ⅰ型蛋白在胶质瘤中的表达及其临床意义  

Expression and significance of heme oxygenase I in gliomas

作　　者：欧阳乐平[1] 李凯舒 刘家豪[1] 何明亮[1] 蔡望青[1] 邓跃飞[1] 刘安民[1] 

机构地区：[1]中山大学孙逸仙纪念医院神经外科,广州510120 [2]清远市人民医院神经外科,511515

出　　处：《中华神经医学杂志》2017年第6期559-563,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（81672507）

摘　　要：目的研究血红索氧化酶Ⅰ型（HO-1）在胶质瘤组织中的表达及意义。方法（1）选择中山大学孙逸仙纪念医院神经外科自2010年1月至2015年1月采用手术治疗的胶质瘤患者56例。其中WHO分级Ⅰ级4例，Ⅱ级16例，Ⅲ级10例，Ⅳ级26例。将Ⅰ、Ⅱ级胶质瘤分为低级别胶质瘤组，将Ⅲ、Ⅳ级胶质瘤分为高级别胶质瘤组。免疫组化染色检测胶质瘤中HO-1的表达。（2）采用R语言survival工具包分析癌症基金组图谱（TCGA）数据库中1107例胶质瘤患者的预后与HO-1蛋白表达的关系。结果（1）免疫组化染色结果显示高级别胶质瘤中HO-1高表达率（75％）高于低级别胶质瘤（30％），差异有统计学意义（P〈0．05）。高级别胶质瘤中HO-1的表达水平高于低级别胶质瘤．差异有统计学意（P〈0．05）。用HO-1评分绘制ROC曲线，曲线下面积为0．747（P=0．002），说明HO-1的表达水平对胶质瘤分级的判断有参考价值。Log-rank检验显示HO-1低表达组患者的累积生存率高于HO-1高表达组，差异有统计学意义（P〈0．05）。（2）TCGA数据库中HO-1低表达组患者的同期生存率高于HO-1高表达组，差异有统计学意义（P〈0．05）。结论胶质瘤中H0-1蛋白表达水平与胶质瘤恶性程度及预后相关，有望作为反映胶质瘤诊断及治疗的新生物学指标，并成为胶质瘤治疗和研究的新靶点。Objective To investigate the expression and biological value ofheme oxygenase I （HO-1） in gliomas. Methods Fifty-six patients with gliomas, admitted to and accepted surgery in our hospital from January 2010 to January 2015, were chosen in our study; WHO grade I was noted in 4 patients, grade II in 16, grade III in 10, and grade IV in 26 patients; patients with grade I and II were as low-grade glioma group and patients with grade HI and IV were as high-grade glioma group. The HO-1 expression in the two groups was detected by immunohistochemistry. R-langrage survival tool was used to analyze the relation between HO-1 expression and prognosis of 1107 patients with gliomas selected from The Cancer Genome Atlas （TCGA） database. Results Significant differences of HO-1 expression were observed in different grades ofgliomas （P〈0.05）, and HO-1 high-expression rate in the high-grade gliomas （75%） was significantly higher than that in the low-grade group （30%, P〈0.05）. HO-1 protein expression level in the high-grade gliomas was significantly higher than that in the low-grade group （P〈0.05）. Moreover, area under the curve （AUC） of the receiver operating characteristic curve was suggested that the HO-1 could be an ideal determine factor （AUC=0.747, P=0.002）. Log rank analysis indicated that the accumulate survival rate in patients with low HO-1 expression was significantly higherthan that in patients with high HO-1 expression （P〈0.05）. TCGA database analysis showed that simultaneous survival rate in patients with low HO-1 expression was significantly higher than that in patients with high HO-1 expression （P〈0.05）. Conclusion Expression of HO-1 is correlated with the malignant degrees and prognoses of gliomas, and it has potential to be a novel biological marker for the diagnosis and treatment ofgliomas; furthermore, HO-1 could also be a target for the study and treatment of gliomas.

关 键 词：血红素氧化酶Ⅰ型 神经胶质瘤 恶性程度 预后 

分 类 号：R739.41[医药卫生—肿瘤]