宫颈上皮内瘤变组织中高危型HPV感染对凋亡、增殖基因表达的影响  

Effect of high risk HPV infection on the genes expression of apoptosis and proliferation in cervical intraepithelial neoplasia

作　　者：陶旻枫[1] 王小榕[2] TAO Minfeng WANG Xiaorong(Heahh Department, Beijing Maternal and Child Health Care Hospital, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China Department of Perinatal Medicine, Beijing Maternal and Child Health Care Hospital, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China)

机构地区：[1]首都医科大学附属北京妇产医院北京妇幼保健院保健部,北京100026 [2]首都医科大学附属北京妇产医院北京妇幼保健院围产医学部,北京100026

出　　处：《中国医药导报》2017年第17期80-83,91,共5页China Medical Herald

基　　金：中国疾病预防控制中心妇幼保健中心合生元母婴营养与健康研究项目(2015FYH004)

摘　　要：目的研究宫颈上皮内瘤变(CIN)组织中高危型HPV感染对凋亡、增殖基因表达的影响。方法选择2013年5月~2016年10月在首都医科大学附属北京妇产医院北京妇幼保健院进行活检的CIN组织和炎性病变组织,分别作为CIN组和对照组。测定高危型HPV感染情况,根据HPV-DNA的RLU/CO将CIN组织分为<1 RLU/CO组、1~100 RLU/CO组、>100~1000 RLU/CO组、>1000 RLU/CO组。同时,测定宫颈组织中细胞周期蛋白及其激酶、凋亡抑制蛋白的表达量。结果CIN组宫颈组织中cyclinB1、cyclinD1、cyclinE、CDK4、CDK6、Apollon、c-IAP1、Survivin、Livin mRNA表达量显著高于对照组,差异有统计学意义(P<0.05)。1~100 RLU/CO组、>100~1000 RLU/CO组、>1000 RLU/CO组CIN组织中cyclinB1、cyclinD1、cyclinE、CDK4、CDK6、Apollon、c-IAP1、Survivin、Livin mRNA表达量显著高于<1 RLU/CO组,且HPV-DNA的RLU/CO越高,cyclinB1、cyclinD1、cyclinE、CDK4、CDK6、Apollon、c-IAP1、Survivin、Livin mRNA表达量越高,差异有统计学意义(P<0.05)。结论宫颈上皮内瘤变组织中高危型HPV感染能够增加细胞周期蛋白及其激酶、凋亡抑制蛋白的基因表达量。Objective To study the effect of high risk HPV infection on the expression of apoptosis and proliferation genes in cervical intraepithelial neoplasia （CIN）. Methods CIN tissues and inflammatory lesions tissues reeeived biopsy in Beijing Maternal and Child Health Care Hospital, Beijing Obstetrics and Gynecology Hospital, Capital Medical University from May 2013 to December 2016 were collected, respectively enrolled in CIN group and control group. CIN tissues were divided into 〈1 RLU/CO group, 1-100 RLU/CO group, 〉100-1000 RLU/CO group, 〉1000 RLU/CO group according to HPV-DNA RLU/CO. Meanwhile, cell cycle protein and its kinase, inhibitor of apoptosis proteins in cervical tissue were determined. Results Expression levels of cyelinB1, cyclinD1, cyclinE, CDK4, CDK6, Apollon, e-IAP1, Survivin, Livin mRNA in cervical tissue of CIN group were significantly higher than those of con- trol group, with statistically significant differences （P 〈 0.05）. Expression levels of eyclinB1, eyclinD1, cyclinE, CDK4, CDK6, Apollon, e-IAP1, Survivin, Livin mRNA in CIN cervical tissue of 1-100 RLU/CO group, 〉100-1000 RLU/ CO group, 〉1000 RLU/CO group were significantly higher than those of the 〈1 RLU/CO group, and the higher the HPV-DNA RLU/CO, the higher the expression of cyelinB1, eyclinD1, cyclinE, CDK4, CDK6, Apollon, c-IAPI, Survivin, Livin mRNA, with statistically significant differences （P 〈 0.05）. Conclusion High risk HPV infection in cervical intraepithelial neoplasia can increase the expression of cyclin and its kinase and inhibitor ＇ of apoptosis protein genes.

关 键 词：宫颈上皮内瘤变 高危型人乳头瘤病毒 细胞周期 凋亡抑制蛋白 

分 类 号：R737.33[医药卫生—肿瘤]