HLJ1在肝癌细胞系中的表达及其对肝癌SK-Hep1细胞生物学行为的影响  

Expression of HLJ1 in hepatocellular carcinoma cell lines and its biologic function in SK-Hep1

作　　者：谭茜茜[1] 汤华[1] 

机构地区：[1]重庆医科大学感染性疾病分子生物学教育部重点实验室,重庆400016

出　　处：《重庆医科大学学报》2017年第7期828-833,共6页Journal of Chongqing Medical University

基　　金：重庆医科大学培育基金资助项目(编号:201416);重庆市渝中区科技计划项目基础与前沿研究计划资助项目(编号:20140122)

摘　　要：目的:研究人肝脏DnaJ-like蛋白(human liver Dna J-like protein,HLJ1)在肝癌细胞中的表达,探讨HLJ1对肝癌细胞SK-Hep1生物学行为的影响。方法:首先,qRT-PCR和Western blot检测HLJ1在肝癌细胞中的表达情况。其次,过表达或干扰HLJ1后转染SK-Hep1细胞株,MTS和克隆形成实验检测细胞增殖能力的变化;流式细胞术检测细胞早期凋亡率;划痕实验检测细胞迁移能力;Transwell小室检测细胞侵袭能力的变化。结果:qRT-PCR和Western blot结果显示HLJ1的mRNA水平和蛋白水平在3种肝癌细胞系中均呈低表达。MTS和克隆形成结果显示HLJ1能抑制肝癌细胞的增殖。流式细胞术结果显示HLJ1能促进细胞早期凋亡。划痕和Transwell实验显示,HLJ1能抑制肝癌细胞的迁移和侵袭。结论:HLJ1在肝癌细胞系中低表达,且能抑制SK-Hep1细胞增殖、迁移和侵袭,促进细胞凋亡。Objective：To investigate the expression of HLJ1 in hepatocellular carcinoma （HCC） cells and to explore the effect of HLJ1 on the biological behavior in SK-Heplcells. Methods：The expressions of HLJ1 mRNA and protein were determined by qRT-PCR and Western blot in hepatocellular carcinoma cells（SMMC-7721, SK-Hepl and HepG2）. MTS, Colony formation and apoptosis assay were performed to determine the cell proliferation and apoptosis;wound healing and Transwell assay were used to detect the migration and invasion in SK-Hepl. Results：qRT-PCR showed HLJ1 mRNA expression were lower in HCC cell lines, and HLJ1 protein level was also lower in HCC cell lines;MTS and Colony formation assay indicated HLJ1 decreased HCC cells proliferation. FCM showed that HLJ1 promoted cell apoptosis. Wound healing and Transwell assay revealed HLJ1 inhibited HCC cell migration. Conclusion： HLJ1 is low expression in HCC cell lines, which could inhibit the proliferation, migration and invasion,while promote cell apoptosis in SK-Hep1.

关 键 词：肝癌 SK-Hep1 人肝脏DnaJ-like蛋白 生物学行为 

分 类 号：R735.7[医药卫生—肿瘤]