爱先蓝和细胞角蛋白双重染色技术在泪腺腺样囊性癌诊断中的应用  

Application of Alcian Blue and cytokeratin double staining technique in adenoid cystic carcinoma

作　　者：高欢欢[1] 林健贤[1] 李扬杵 张文忻[1] 陈丽红[1] 唐丽娟[1] 张平[1] GAO Huanhuan LIN Jianxian LI Yangchu ZHANG Wenxin CHEN Lihong TANG Lijuan ZHANG Ping(Clinical Pathology Department, Zhongshan Ophthalmic Center, Sun Yat-sen University, State Key Laboratory of Ophthalmology, Guangzhou 510060, China.)

机构地区：[1]中山大学中山眼科中心临床病理科,眼科学国家重点实验室,广州510060

出　　处：《Eye Science》2017年第2期64-67,共4页眼科学报（英文版）

基　　金：国家自然科学基金(30371515)~~

摘　　要：目的:探讨爱先蓝(Alcian Blue,AB)和细胞角蛋白(cytokeratin,CK)双重染色技术在泪腺腺样囊性癌组织中的应用,提高泪腺腺样囊性癌染色效率。方法:选取中山大学中山眼科中心临床病理科2015年1月至2017年1月期间诊断为泪腺腺样囊性癌病例标本23例,在同一张切片上先进行AB染色,再进行CK染色,观察染色效果。结果:23例泪腺腺样囊性癌标本组织中黏液物质全部呈蓝色;癌细胞胞质CK阳性,呈棕黄色。结论:AB和CK双重染色方法稳定,颜色对比鲜明,能够良好显示癌细胞及黏液的关系,并且比分开的两次单种染色省时、经济。Objective： To investigate the application of Alcian Blue （AB） and cytokeratin （CK） double staining technique in adenoid cystic carcinoma to improve the staining efficienc）, Methods： Twenty-three specimens of adenoid cystic carcinoma from January 2015 to January 2017 in the Clinical Pathology Department of Zhongshan Ophthalmic Center, Sun Yat-sen University were stained with AB first and CK then on the same slide. Results： Mucinous substance showed blue. And the cytoplasm of the cancer cells presented with brown yellow in all the specimens. Conclusion： The method of double staining with AB and CK is stable and displays bright color contrast. It can effectively reveal the relationship between the mucus and cancer cells. It proves to be more time-saving and economical than the individual staining of AB and CK respectively.

关 键 词：双重染色 泪腺腺样囊性癌 爱先蓝 细胞角蛋白 

分 类 号：R739.7[医药卫生—肿瘤]