秦岭中草药黄芩中黄酮类化合物的分离及其α-葡萄糖苷酶抑制活性研究  

Study on the flavonoids fromScutellaria Baicalensis Georgi in the Qinling Mountains and theirα-glucosidase inhibitory activities

作　　者：林利彬 严铁宁[2] 马琳[1] 肖健[1] 王晓玲[1] 

机构地区：[1]宝鸡文理学院化学化工学院陕西省植物化学重点实验室,陕西宝鸡721013 [2]宝鸡职业技术学院化学工程系,陕西宝鸡721013

出　　处：《宝鸡文理学院学报（自然科学版）》2017年第2期42-46,共5页Journal of Baoji University of Arts and Sciences(Natural Science Edition)

基　　金：教育厅重点项目(13JS005);陕西省科技厅基础研究计划(2014JM2058)

摘　　要：目的分离鉴定秦岭中草药黄芩中的黄酮类物质并研究其对α-葡萄糖苷酶的抑制活性。方法植物原料用溶剂提取,常压硅胶柱色谱及制备液相色谱分离,高分辨质谱及核磁共振波谱分析确定化合物分子结构,PNPG法测定化合物的α-葡萄糖苷酶的抑制活性。结果分离鉴定了8种黄酮类化合物,分别是汉黄芩素(1)、黄芩素(2)、汉黄芩苷(3)、千层纸素A-7-O-葡萄糖酸苷(4)、白杨素-7-O-葡萄糖酸苷(5)、黄芩苷(6)、黄芩素-6-O-葡萄糖酸苷(7)和去甲汉黄芩素-8-O-葡萄糖酸苷(8),活性实验表明化合物1和7表现出较好的α-葡萄糖苷酶的抑制活性,其IC_(50)分别为0.13mmol/L和0.25mmol/L,稍强于阳性对照阿卡波糖(IC_(50):0.42mmol/L)。结论秦岭中草药黄芩中某些黄酮类物质能够有效抑制α-葡萄糖苷酶,具有与阿卡波糖相当的降糖作用。Purposes--To study the flavonoids from Scutellaria baicalensis Georgi in the Qinling Mountains, Baoji, Shaanxi, China and their α-glucosidase inhibitory activities. Methods--Solvent extract and different chromatographies were used to separate the compounds; spectra techniques such as NMR and HR-ESI-MS were utilized to elucidate their structures; and PNPG method was adopted to screen the α-glucosidase inhibitory activities of the isolated compounds. Results--Eight flavonoids compounds were isolated and identified as wogonin （1）, baicalein （2）, wogonoside （3）, oroxylin A-7- O-glucuronide （4）, chrysin-7-O-glucuronide （5）, baicalin （6）, baicalein-6-O-glucuronide （7） and norwogonin-8-O-glucuronide （8）. The bioassay experiment revealed that compounds 1 and 7 showed a little stronger α-glucosidase inhibitory activity with IC50 values of 0. 13 mmol/L and 0.25 mmol/L, respectively （IC50 of acarbose was 0. 42 mmol/L）. Conclusion--Some flavonoids from Scutellaria baicalensis Georgi showed the comparable ability with acarbose in inhibiting the α-glucosidase.

关 键 词：黄芩 黄酮类化合物 α-葡萄糖苷酶的抑制活性 

分 类 号：O651[理学—分析化学]