PP2CE在人原发性肝癌中的表达及其临床意义  

Expression of PP2CE in Human Hepatocellular Carcinoma Tissues and the Clinical Significance

作　　者：徐卫华 黄焕军[2] 

机构地区：[1]湖北省孝感市孝昌县第一人民医院消化内科,孝感432900 [2]华中科技大学同济医学院附属同济医院消化内科,武汉430030

出　　处：《华中科技大学学报（医学版）》2017年第3期327-331,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨PP2CE在人原发性肝癌组织中的表达、亚细胞定位及其与临床病理特征的关系。方法收集武汉同济医院2010~2015年手术切除的原发性肝癌(HCC)及其对照的癌旁组织标本50例。采用荧光定量PCR法和免疫组织化学法分别检测PP2CE在肝癌及癌旁组织中mRNA和蛋白表达水平,分析其与临床病理资料之间的关系。免疫荧光法检测PP2CE在肝癌及癌旁组织中的亚细胞定位。结果肝癌中PP2CE mRNA和蛋白表达水平较其对应的癌旁组织明显减少(均P<0.05),通过分析PP2CE免疫组化结果与临床病理特征的关系发现,PP2CE减少与肝癌大小、分化程度、门静脉侵犯和临床分期相关(均P<0.05)。免疫荧光检测表明PP2CE主要表达于细胞质和核周。结论PP2CE在HCC中表达减少,其可能参与抑制肝癌的发生和发展。Objective To investigate expression of PP2 CE in human hepatocellular carcinoma（HCC）tissues and the clinical pathological significance,and test its sub-cellular localization in liver cells and HCC cells.Methods A total of 50 samples of HCC tissues and their para-carcinoma tissues were collected in Tongji Hospital（Wuhan）.PP2 CE mRNA and protein levels were detected in HCC tissues and their para-carcinoma tissues by qRT-PCR and immunohistochemistry method.Immunofluorescent assay was performed to test the sub-cellular localization of PP2 CE in the cells.Results PP2 CE mRNA and protein expression levels were significantly decreased in HCC tissues as compared with those in para-carcinoma tissues（both P〈0.05）.Immunohistochemistry analysis found that PP2 CE expression was associated with tumor size,differentiated degree,portal vein invasion and clinical stage（both P〈0.05）.Immunofluorescent assay suggested that PP2 CE was mainly located in the cytoplasm and perinuclear area.Conclusion PP2 CE is reduced in HCC and may be implicated in the development and progression of HCC.

关 键 词：PP2CE 肝细胞癌 亚细胞定位 转移 

分 类 号：R735.7[医药卫生—肿瘤]