MicroRNA-638在骨肉瘤中表达下调及对其细胞增殖的影响  

Effect of MicroRNA-638 expression on the proliferation of human osteosarcoma cells

作　　者：陈斌[1] 符勇[1] 夏雪[1] 郭伟明[1] 金浩成 王晓旭[1] 

机构地区：[1]南华大学附属第二医院骨科,湖南衡阳421001

出　　处：《中南医学科学杂志》2017年第4期341-345,363,共6页Medical Science Journal of Central South China

基　　金：湖南省教育厅资助项目(15C1215)

摘　　要：目的探讨miR-638在人骨肉瘤(OS)细胞增殖行为中的影响。方法通过实时定量聚合酶链反应(qRT-PCR)检测miRNA-638在人OS组织及癌旁组织、人OS细胞株(MG63、U2OS、HOS、SaOS2)和正常人成骨NHOst细胞株中的表达;用LipofectamineTM2000转染miR-638模拟物或抑制物,细胞增殖检测试剂盒(CCK-8)检测转染后的吸光光度值,平板克隆形成实验检测菌落数量。结果 miR-638在各组细胞中均有表达;OS细胞株中miR-638的表达明显下调(P<0.05);高表达miR-638能抑制OS MG63和U2OS细胞的增殖(P<0.05)。结论miR-638高表达能抑制OS细胞的增殖。Objective To investigate the role of miR-638 in biological behavior of osteosarcoma cell.MethodsThe expression of miR-638 in osteosarcoma MG63 and U2OS cells was detected by RT-PCR.miRNA-638 mimics （inhibhors） were transfected into MG63 and U2OS by using LipofectamineTM2000 to upregulate（deregulate） the expression of miRNA-638.The absorbance value in 24 h,48 h,96 h and 72 h following the transfection was detected by CCK-8.And colony forming assay was used to detect colony counts over 30 cells after 2 weeks.ResultsMiR-638 expressed in each group.The expression of miR-638 was down regulated （P〈0.001） in the 36 groups （56.25%） compared with the corresponding non cancerous tissues.Mir-638 expression can significantly reduce the MG63 and U2OS cells growth rate （P〈0.05）,on the contrary,the suppression of miR-638 expression significantly promoted the growth of cells of U2OS and MG63 （P〈0.05）.ConclusionsHigh expression of miR-638 significantly inhibits the proliferation of osteosarcoma cells.MiR-638 may become a new target for the early diagnosis and treatment of osteosarcoma.

关 键 词：骨肉瘤 MG63 U2OS MicroRNA-638 细胞增殖 

分 类 号：R738.1[医药卫生—肿瘤]