腺病毒过表达TSLC1对宫颈癌细胞增殖侵袭能力和血管新生能力的影响  

Effects of adenovirus overexpression of TSLC1 on proliferation,invasion and angiogenesis of cervical cancer cells

作　　者：程凡玲 张红莉[2] 

机构地区：[1]延安医疗集团总医院宜川县人民医院分院妇产科,陕西延安716200 [2]延安大学附属医院妇产科,陕西延安716000

出　　处：《海南医学院学报》2017年第10期1315-1318,共4页Journal of Hainan Medical University

基　　金：延安市科技局(2015HM-01-01)~~

摘　　要：目的:研究腺病毒过表达肺癌肿瘤抑制因子-1(tumor suppressor in lung cancer 1,TSLC1)对宫颈癌细胞增殖侵袭能力和血管新生能力的影响。方法:培养Caski宫颈癌细胞株并分为空白对照组、Ad-RFP组、Ad-TSLC1组,分别用不含腺病毒及血清的DMEM、对照腺病毒Ad-RFP、TSLC1过表达的重组腺病毒Ad-TSLC1处理,12、24h后分别测定增殖、侵袭、血管新生基因的表达量。结果:AdTSLC1组细胞内HERC4、CyclinD1、Bcl-2、S100A6、N-cadherin、Snail、Twist、EFEMP1、VEGF、Jagged1的mRNA表达量显著低于空白对照组、Ad-RFP组,Ad-RFP组细胞内HERC4、CyclinD1、Bcl-2、S100A6、N-cadherin、Snail、Twist、EFEMP1、VEGF、Jagged1的mRNA表达量与空白对照组比较差异无统计学意义(P>0.05)。结论:腺病毒过表达TSLC1能够抑制宫颈癌细胞的增殖侵袭及血管新生。Objective： To study the effect of overexpression of TSLC1 on proliferation, invasion and angiogenesis of cervical cancer cells.Methods： Caski cervical cancer cells were cultured and divided into blank control group, Ad-RFP group, Ad-TSLC1 group, respectively transfected with DMEM without adenovirus and serum, control adenovirus Ad-RFP, recombinant adenovirus Ad-TSLC1.Of 12 hours and 24 hours later, the expression of proliferation, invasion, and angiogenesis gene were determined.Results： mRNA expression of CyclinD1, Bcl-2, HERC4 cells, S100A6, N-cadherin, Snail, Twist, EFEMP1, VEGF, Jagged1 in Ad-TSLC1 group were significantly lower than the control group and Ad-RFP group;mRNA expression of CyclinD1, Bcl-2, HERC4 cells, S100A6, N-cadherin, Snail, Twist, EFEMP1, VEGF, Jagged1 in Ad-RFP group and control had no significant difference.Conclusions： Overexpression of TSLC1 can inhibit the proliferation, invasion and angiogenesis of cervical cancer cells.

关 键 词：宫颈癌 肺癌肿瘤抑制因子-1 增殖 侵袭 血管新生 

分 类 号：R737.33[医药卫生—肿瘤]