人肝细胞癌中miR-23a和XIAP的表达及意义  

Expression of miR-23a and XIAP in hepatocellular carcinoma and their significance

作　　者：马伟[1] 吴文涌[1] 吴正升[2] 汪树静 余昌俊[1] 

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022 [2]安徽医科大学病理学教研室,合肥230032

出　　处：《安徽医科大学学报》2017年第7期1008-1011,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81572305)

摘　　要：目的探讨miR-23a和X连锁凋亡抑制蛋白(XIAP)在人肝细胞癌(HCC)中的表达、相互关系及临床病理意义。方法建立组织微阵列,采用原位分子杂交方法分别检测150例人HCC和136例相对应的癌旁组织中miR-23a的变化;利用免疫组化两步法检测XIAP蛋白和Ki-67蛋白的表达水平。结果 HCC组织和癌旁组织中miR-23a的高表达率分别为48.7%(73/150)和77.2%(105/136),两者差异有统计学意义(P=0.001),XIAP蛋白在HCC和癌旁组织中的高表达率分别为25.3%(38/150)和22.1%(30/136),两者差异无统计学意义;miR-23a高表达率在患者中男性高于女性(P=0.037)、HBs Ag阳性高于HBs Ag阴性患者(P=0.022)、大肝癌高于小肝癌(P=0.021)、Ⅰ~Ⅱ期高于Ⅲ~Ⅳ期(P=0.002);而XIAP蛋白高表达在患者中小肝癌高于大肝癌(P=0.001)、Ⅰ~Ⅱ期高于Ⅲ~Ⅳ期(P=0.009)。此外两者均与患者年龄、是否合并肝硬化、组织学分级无关。经Pearson相关检验,miR-23a和XIAP蛋白在HCC中的表达呈显著负相关性(rs=-0.308,P<0.01)。结论 miR-23a和XIAP可能参与HCC的发生与发展。Objective To investigate the expression and correlation of miR-23 a and X-linked inhibitor of apoptosis protein（ XIAP） in hepatocellular carcinoma（ HCC） and their clinicopathological significance. Methods The expressions of miR-23 a in 150 HCC and 136 adjacent tissues were detected by in situ hybridization.The expressions of XIAP and Ki-67 protein were detected by immunohistochemistry two-step method. Results The high expression rates of miR-23 a were 48. 7%（73/150） and 77. 2%（105/136） in HCC tissues and adjacent non-cancerous tissues respectively,there was significant difference between the two groups（ P = 0. 001）.The high expression rates of XIAP were 25. 3%（38/150） and 22. 1%（30/136） in HCC tissues and adjacent non-cancerous tissues respectively,there was no significant difference between the two groups. The expression rate of miR-23 a was higher in male than in female（ P = 0. 037）,HBs Ag positive was higher than that of HBs Ag negative patients（ P = 0. 022）,large hepatocellular carcinoma was higher than that of small hepatocellular carcinoma（ P = 0. 021）,and stageⅠ ~ Ⅱwas higher than that of stageⅢ ~ Ⅳ（ P = 0. 002）. While the high expression of XIAP protein in patients with small hepatocellular carcinoma was higher than that of large hepatocellular carcinoma（ P = 0. 001）,theⅠ ~ Ⅱ stage was higher than Ⅲ~ Ⅳstage（ P = 0. 009）. In addition,both of them had no correlation with age,liver cirrhosis and histological grading.The expression of miR-23 a and XIAP protein in hepatocellular carcinoma was negatively correlated by Pearson＇s correlation（ rs=-0. 308,P〈0. 01）.Conclusion miR-23 a and XIAP may be involved in the development and progression of HCC.

关 键 词：肝细胞癌 miR-23a XIAP 免疫组化 原位分子杂交 

分 类 号：R735.7[医药卫生—肿瘤]