洛伐他汀对抗AβOs引起的SH-SY5Y细胞毒性作用  

Antagonism of Lovastatin on Cytotoxicity in SH-SY5Y Cells Induced by β-Amyloid Peptide Oligomers

作　　者：谭龙春 吴昌学[1,2] 赵亮[3] 董阳婷 刘仙红 官志忠[1,2,4] 

机构地区：[1]贵州医科大学分子生物学重点实验室,贵州贵阳550004 [2]贵州医科大学地方病与少数民族性疾病教育部重点实验室,贵州贵阳550004 [3]贵州医科大学微生物学教研室,贵州贵阳550004 [4]贵州医科大学病理学教研室,贵州贵阳550004

出　　处：《贵州医科大学学报》2017年第6期650-654,660,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81260173);教育部"长江学者和创新团队发展计划资助"(IRT13058);贵州省科技计划[黔科合重大专项字(2014)6008号];贵州省创新计划项目[黔教合协同创新中心(2014)06]

摘　　要：目的:观察洛伐他汀对抗Aβ寡聚体(AβOs)引起的SH-SY5Y细胞毒性作用。方法:生长至80%~90%并用无血清培养基培养12 h的SH-SY5Y细胞分为对照组、洛伐他汀处理组(0.1μmol/L洛伐他汀处理24 h)、AβOs处理组(0.5μmol/L AβOs处理48 h)和洛伐他汀加AβOs处理组(0.1μmol/L洛伐他汀处理24 h,再加入0.5μmol/L AβOs继续处理48 h),采用CCK-8法检测各组细胞活性,使用相应的试剂盒检测细胞超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量。结果:0.5μmol/L AβOs处理SHSY5Y细胞48 h后,与对照组相比,SH-SY5Y细胞活性、SOD及GSH-Px活性显著降低,MDA含量明显增加(P<0.05);在0.5μmol/L AβOs处理SH-SY5Y细胞前,预先用0.1μmol/L洛伐他汀处理细胞24 h,可减弱AβOs引起的细胞活性、SOD和GSH-Px活性及MDA含量的改变(P<0.05)。结论:洛伐他汀可对抗AβOs引起的SHSY5Y细胞毒性作用,其保护机制可能与抑制氧化应激有关。Objective： To investigate the effect of lovastatin on cytotoxicity in SH-SY5 Y cells induced by β-Amyloid peptide oligomers（ AβOs）. Methods： When SH-SY5 Y cells were grown to 80% ～90% and cultured in serum-free medium for 12 h,they were divided into control group,lovastatin group（ 0. 1 μmol/L lovastatin for 24 h）,AβOs group（ 0. 5 μmol/L βOs for 48 h） and lovastatin ＋AβOs group（ 0. 1 μmol/L lovastatin treated with for 24 h,and then treated with 0. 5 μmol/L AβOs for 48 h）. The cell viability was measured by CCK-8 assay,superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-PX） activities and malondialdehyde（ MDA） content were measured by appropriate biochemical methods. Results： As compared with control group,the cell viability,SOD and GSH-PX activities were significant decreased,while MDA content was significantly increased after SHSY5 Y cells treated with 0. 5 μmol/L AβOs for 48 h（ P〈0. 05）. When SH-SY5 Y cells were treatedwith 0. 1 μmol/L lovastatin for 24 h before treatments of AβOs,these cytotoxicity resulted by AβOs indicated as cell viability,SOD and GSH-PX activity and MDA content were all significantly attenuated（ P〈0. 05）. Conclusions： Lovastatin could antagonize the cytotoxicity in SH-SY5 Y cells caused by AβOs,and the mechanism might relate to the inhibition of oxidative stress.

关 键 词：阿尔茨海默病 洛伐他汀 AΒ寡聚体 细胞毒性 氧化应激 SH-SY5Y细胞 

分 类 号：R741[医药卫生—神经病学与精神病学] R961[医药卫生—临床医学]