C20rf40蛋白在鼻咽癌中的表达及意义  

Expression of C2orf40 protein and its clinical significance in human nasopharyngeal carcinoma

作　　者：彭琳[1] 黄裔腾[3] 陈炯玉[2] 洪超群[2] 吴晓[2] Peng Lin Huang Yiteng Chen Jiongyu Hong Chaoqun Wu Xiao(Clinical Laboratory, Cancer Hospital of Shantou University Medical College, Shantou 515041, China)

机构地区：[1]汕头大学医学院附属肿瘤医院检验科,515041 [2]汕头大学医学院附属肿瘤医院肿瘤研究实验室,515041 [3]汕头大学医学院第一附属医院健康管理中心

出　　处：《国际肿瘤学杂志》2017年第6期406-410,共5页Journal of International Oncology

基　　金：国家自然科学基金（81602886）;广东省医学科研基金（A2015383）;汕头市科技计划（汕府科[2015]132号-113）

摘　　要：目的探讨C2orf40蛋白在鼻咽癌组织和细胞中的表达及其与细胞分化、临床病理特征的关系。方法收集2001年1月至2003年12月在汕头大学医学院附属肿瘤医院诊断为鼻咽癌的122例病理活检组织石蜡标本及其病历资料，另外收集25例慢性鼻咽炎的石蜡标本作为对照。采用免疫组织化学法检测C2orf40蛋白表达情况，同时分析C2orf40表达水平与临床病理特征的关系。体外实验应用Western blotting对鼻咽癌细胞株CNE1、CNE2和C6661进行C2orf40蛋白表达的测定，分析C2orf40表达与细胞分化程度的关系。结果C2orf40在对照组的阳性率为96.0%（24/25），且88.0%为高表达，病例组阳性率为58.2%（71/122），但82.0%为低表达，二者差异有统计学意义（U=255.500，P＜0.001）。C2orf40表达与淋巴结转移分期（N分期）和临床分期呈负相关（r=-0.058，P＜0.001；r=-0.202，P=0.026）。C2orf40在高分化鼻咽癌细胞株CNE1中呈高表达，在低分化鼻咽癌细胞株CNE2和C6661中呈低表达。结论C2orf40蛋白表达下调与肿瘤细胞分化、淋巴结转移和临床分期相关，是鼻咽癌发生、发展的分子事件之一，可能成为抗肿瘤治疗的潜在靶点。ObjectiveTo evaluate the protein expression of chromosome 2 open reading frame 40 （C2orf40） in nasopharyngeal carcinoma （NPC） tissues and cells, and to explore its association with cell differentiation and clinicopathological features. MethodsA total of 122 patients with NPC between January 2001 and December 2003 were enrolled in Cancer Hospital of Shantou University Medical College. The paraffinembedded tissue sections and medical records were collected. Twentyfive samples with chronic nasopharyngitis were used as controls. Tumor and control tissues from biopsies underwent immunohistochemical staining for C2orf40. C2orf40 expression was analyzed with clinicopathological variables. Besides, the protein expressions of C2orf40 were measured by Western blotting in three NPC cell lines, including CNE1, CNE2 and C6661. ResultsNinetysix percent （24/25） of control tissues showed positive expression, among which 88.0% showed dense staining. Otherwise, only 58.3% （71/122） of NPC samples were positive for C2orf40 protein and 82.0% showed weak staining. There was significantly difference between the two groups （U=255.500, P〈0.001）. It was inversely related to lymph nodes status （r=-0.058, P〈0.001） and clinical stage （r=-0.202, P=0.026） by Spearman rank correlation test. In vitro, higher level of C2orf40 protein was found in well differentiated CNE1 cells, while lower levels were found in poorly differentiated cell lines CNE2 and C6661. ConclusionDownregulated C2orf40 expression is correlated with tumor cell differentiation, lymph nodes metastasis and clinical stage, and may be a molecular event in the occurrence and development of NPC. C2orf40 is likely to be a potential target of anticancer therapy.

关 键 词：鼻咽肿瘤 病理学 临床 C2orf40 

分 类 号：R739.63[医药卫生—肿瘤]