CpG-ODN对肺癌A549细胞化疗的协同作用  

CpG-ODN enhances sensitivity to chemotherapy in lung cancer cell line A549

作　　者：王凯玲[1] 费广茹 任涛[1] 

机构地区：[1]同济大学附属东方医院呼吸科,上海200120 [2]安徽省省立医院呼吸科,安徽合肥230001

出　　处：《同济大学学报（医学版）》2017年第3期14-18,24,共6页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(81372347);上海市浦东新区卫生系统重点学科建设项目(PWZx2014-10)

摘　　要：目的研究CpG寡聚脱氧核苷酸(CpG-ODN)对肺癌A549细胞化疗的影响,并探讨其可能的作用机制。方法将肺癌A549细胞分为空白对照组、DDP组、CpG-ODN组、DDP+CpG-ODN组、CpG-ODN+DDP组。采用CCK-8比色法检测细胞的增殖情况,流式细胞仪检测细胞凋亡情况,RT-PCR检测TLR9 mRNA的表达,Western印迹法检测TLR9信号通路中My D88、AP-1的表达。结果 TLR9激活CpG ODN后,能显著提高细胞的增殖能力(P<0.05);CpG-ODN与CpG-ODN+DDP两组细胞生长无明显差异(P>0.05);CpG-ODN+DDP组细胞凋亡率较DDP组低(P<0.05),DDP+CpG-ODN组细胞凋亡率较DDP组明显升高(P<0.05);DDP+CpG-ODN组TLR9 mRNA较DDP组明显升高(P<0.05);DDP+CpG组AP-1蛋白表达上调(P<0.05)。结论 CpG-ODN激活TLR9信号通路后,可协同DDP促进细胞凋亡,提高顺铂化疗的敏感性。Objective To investigate the effect of TLR9 activator CpG-ODN on chemosensitivity of lung cancer A549 cells.Methods Cultured lung cancer A549 cells were divided into control group, DDP group, CpG-ODN group, DDP＋CpG-ODN group, CpG-ODN＋DDP group.Cell proliferation was analyzed with CCK-8 method;cell apoptosis was examined with flow cytometry;the mRNA expression of TLR9 was detected with RT-PCR;and the protein expression of MyD88 and AP-1 was detected with Western blotting.Results The cell proliferation was improved in CpG-ODN groups（ P 〈0.05）;there was no significant difference between CpG-ODN and CpG-ODN＋DDP groups（ P 〉0.05）.The apoptosis rate of CpG-ODN＋DDP was lower than that of DDP（ P 〈0.05）, and the apoptotic rate of DDP＋CpG-ODN was significantly higher than that of DDP（ P 〈0.05）.The expression of TLR9 mRNA in DDP＋CpG-ODN was higher than that in DDP group（ P 〈0.05）.The expression of AP-1 in DDP＋CpG group was up-regulated（ P 〈0.05）.Conclusion The data indicate that the CpG-ODN can increase cell apoptosis and enhance the sensitivity of lung cancer cells to cisplatin chemotherapy.

关 键 词：非小细胞肺癌 CP G寡聚脱氧核苷酸 化学疗法 

分 类 号：R734.2[医药卫生—肿瘤]