局部应用β_1转化生长因子对兔下颌牵张成骨的影响  

Effects of Locally Applied TGF-β_1 on Mandibular Distraction Osteogenesis in Rabbits.

作　　者：王志国[1] 胡静[1] 邹淑娟[1] 王大章[1] 廖运茂[1] 

机构地区：[1]四川大学华西口腔医学院,四川成都610041

出　　处：《口腔医学研究》2002年第4期228-230,共3页Journal of Oral Science Research

基　　金：国家自然科学基金 (编号 :39970 797);教育部留学回国人员科研启动基金资助

摘　　要：目的 :研究外源性 β1转化生长因子对牵张成骨的作用。方法 :将 16只大耳白兔随机分为A、B两组 ,每组8只。在兔双侧下颌骨前部行骨切开术后用自行研制的牵张器延长双侧下颌骨 6mm。从牵张开始第 1天在A组动物牵张区每日注射TGF - β14 0ng ,连续 12d。不注射TGF - β1的B组动物用作对照。在牵张结束后第 2和第 4周分别处死A、B两组各 4只动物 ,取下颌骨标本及牵张区新生骨痂进行X线和组织学检查。结果 :注射TGF - β1的A组动物牵张间隙内新骨形成并不优于B组动物。相反 ,在第 2周时还发现有较多的纤维组织和软骨形成。结论 :导入外源性TGF -Objective:To study the effects of exogenous TGF-β 1 on mandibular distraction osteogenesis.Methods:16 mature rabbits were randomly divided into group A (n=8) and B (n=8).Bilateral mandibular osteotomies were performed ,and the mandibles of 16 rabbits were lengthened 6 mm using a custom-made distractor.Starting with distraction,TGF-β 1 was applied in a dosage of 40 ng/d for 12 days, whereas group B was used as control.4 rabbits were killed respectively at 2 and 4 weeks after completion of distraction in group A or group B.X-Ray examination was taken and the distracted callus was processed for histological analysis.Results:The bone regeneration in group A was not superior to that of group B after mandibular osteodistraction.In contrast, more fibrous tissue and cartilage islands were observed in group A at 2 weeks postdistraction when compared with group B.Conclusions:Exogenous TGF-β 1 may have no beneficial effect on bone formation following mandibular osteodistraction in rabbits.

关 键 词：Β1转化生长因子 兔 下颌牵张成骨 影响 骨再生 

分 类 号：R782.2[医药卫生—口腔医学]