神经病理性疼痛对溃疡性结肠炎大鼠肠道炎症变化及免疫球蛋白浓度的影响  

Effects of neuropathic pain on intestinal pathology and immunoglobulin levels in rats with ulcerative colitis

作　　者：沈玲[1] 邱海波[1] 陈前波[1] 陆智杰[1] 

机构地区：[1]第二军医大学附属东方肝胆外科医院麻醉科,上海市200438

出　　处：《临床麻醉学杂志》2017年第6期592-594,共3页Journal of Clinical Anesthesiology

摘　　要：目的观察脊神经结扎(spinal nerve ligation,SNL)导致的疼痛对溃疡性结肠炎大鼠肠道病理改变及循环中免疫球蛋白浓度的影响。方法健康雄性大鼠36只,体重200~220g,采用随机数字表法分为三组:SNL模型组(SNL组)、假手术组(Sham组)和对照组(Con组),每组12例。建立稳定的疼痛模型后,三组大鼠均予以三硝基苯磺酸(TNBS)诱导产生溃疡性结肠炎。分别于SNL术前、TNBS造模前及造模后第7天和第14天采血检测血清免疫球蛋白(IgG、IgM)浓度变化,并于造模后第7天取病变明显部位的结肠组织观察炎症病理改变。结果三组大鼠术前IgG、IgM浓度差异无统计学意义;脊神经结扎后SNL组血清IgG浓度略低于Sham组,而血清IgM浓度明显低于Sham组(P<0.05);TNBS造模第7天SNL组大鼠血清IgG、IgM浓度升高幅度明显小于Sham组和Con组(P<0.01);TNBS造模第14天SNL组大鼠血清IgG浓度明显低于Sham组及Con组(P<0.05);TNBS造模第7天,与Sham组和Con组比较,SNI组结肠病变范围更为广泛,炎症程度更为严重。结论神经病理性疼痛抑制机体体液免疫,使血清IgM、IgG浓度降低,从而加重肠道炎症病理改变程度。Objective To investigate the effects of spinal nerve ligation（SNL）on the changes of intestinal pathology and the levels of serum immunoglobulin in rats with ulcerative colitis.Methods Thirty-six healthy male rats,weighing 200-220 g,were randomly divided into three groups using the random number table method：spinal nerve ligation model group（group SNL）,sham-operationgroup（group Sham）and non-operatedcontrol group（group Con）,12 in each group.After the establishment of SNL models,the three groups were given trinitro-benzene-sulfonic acid（TNBS）to induce ulcerative colitis.The serum level of immunoglobulin（IgG,IgM）were measured on the day before SNL,before TNBS modeling and 7,14 dafter TNBS modeling,respectively.Besides,the intestinal pathology were observed on 7dafter TNBS modeling.Results The basic values of IgM and IgG before operation were not statistically different among three groups.The level of IgG in group SNL was lower than that in group Sham after SNL operation with no significant difference,but the level of IgM was significantly lower than that of group Sham（P〈0.05）.On 7dafter TNBS modeling,both levels of IgG and IgM in group SNL were significantly lower than those in group Sham and group Con（P〈0.01）.On 14 d after TNBS modeling,the level of IgG in group SNL was significantly lower than that in group Sham and group Con（P〈0.05）.Furthermore,the colon lesions were more extensive and the inflammation was more serious in group SNL than those in group Sham and group Con on 7 d after TNBS modeling.Conclusion Neuropathic pain suppress immune activities,reduces the serum level of IgM and IgG and aggravates intestinal inflammation caused by TNBS.

关 键 词：神经病理性疼痛 溃疡性结肠炎 免疫应答 

分 类 号：R574.62[医药卫生—消化系统]