苹果MdRGL3a基因的功能分析  

Function Analysis of MdRGL3a Gene in Malus × domestica

作　　者：任怡然[1] 赵强[1] 由春香[1] 郝玉金[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,国家苹果工程技术研究中心,山东泰安271018

出　　处：《园艺学报》2017年第6期1021-1028,共8页Acta Horticulturae Sinica

基　　金：农业部公益性行业科研专项(201203075-3);教育部“创新团队发展计划”项目(IRT15R42);山东省现代农业产业技术体系创新团队项目(SDAIT-06-03)

摘　　要：为进一步揭示苹果(Malus×domestica)中GA信号转导机制以及MdDELLAs的生理功能,以‘嘎拉’苹果为试材,克隆基因MdRGL3a(GenBank:DQ007887),并通过在拟南芥中过量表达,分析该基因的功能。qRT-PCR结果表明,MdRGL3a在苹果不同组织中都有表达,其中在花和果实中表达量较高。在拟南芥中异位表达MdRGL3a,表现为根长和下胚轴变短,种子萌发较慢,叶片变小,植株矮化。过表达MdRGL3a拟南芥植株对赤霉素介导的生长反应敏感性降低,证明MdRGL3a是GA信号途径中的一个负调控因子。In order to further reveal the mechanism of GA signal transduction in apple（Malus × domestica） and the physiological function of MdDELLAs,a gene named MdRGL3a（Gen Bank：DQ007887）cloned from ＇Royal Gala＇ was analyzed. The function of this gene was analyzed by ectopic expression of MdRGL3 a in Arabidopsis thaliana. Real-time quantitative PCR results showed that MdRGL3 a was expressed in different tissues,and the expression level was higher in flowers and fruits. Ectopic expression of MdRGL3 a in Arabidopsis thaliana reduced response to GA3,which exhibited shorter root and hypocotyl,slower germination,smaller leaves and shorter stalk. The result of Arabidopsis thaliana plants treated with GA3 showed that MdRGL3 a reduced the promotion of GA for plants growth and development,revealing that MdRGL3 a might be a negative regulator of GA signaling pathway.

关 键 词：苹果 MdRGL3a DELLA蛋白 表达分析 功能鉴定 

分 类 号：S661.1[农业科学—果树学]