Inhibition effect of curcumin on UVB-induced secretion of pro-inflammatory cytokines from corneal limbus epithelial cells  

作　　者：Shih-Chun Chao Dan-Ning Hu Joan Roberts Xilun Shen Chia-Yi Lee Chan-Wei Nien Hung-Yu Lin Shih-Chun Chao;Dan-Ning Hu;Joan Roberts;Xilun Shen;Chia-Yi Lee;Chan-Wei Nien;Hung-Yu Lin(Department of Ophthalmology,Show Chwan Memorial Hospital;Department of Electrical and Computer Engineering,National Chiao Tung University;Department of Optometry,Central Taiwan University of Science and Technology;Tissue Culture Center,New York Eye and Ear Infirmary of Mount Sinai;Ichan School of Medicine in Mount Sinai;Fordham University;Institute of Medicine,Chung Shan Medical University;Department of Optometry,Chung Shan Medical University;Department of Optometry,Yuanpei University of Medical Technology)

机构地区：[1]Department of Ophthalmology, Show Chwan Memorial Hospital, Changhua 50093, Taiwan, China [2]Department of Electrical and Computer Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan, China [3]Department of Optometry, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan, China [4]Tissue Culture Center, New York Eye and Ear Infirmary of Mount Sinai, New York, NY 10003, USA [5]Ichan School of Medicine in Mount Sinai, New York, NY 10029, USA [6]Fordharn University, New York, NY 10023, USA [7]Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan, China [8]Department of Optometry, Chung Shan Medical University, Taichung 40201, Taiwan, China [9]Department of Optometry, Yuanpei University of Medical Technology, Hsinchu 30015, Taiwan, China Correspondence to： Hung-Yu Lin. Department of Ophthal- mology, Show Chwan Memorial Hospital, No.526, Sec. 1, Zhongshan Rd., Changhua City, Changhua County 500, Taiwan, China.

出　　处：《International Journal of Ophthalmology(English edition)》2017年第6期827-833,共7页国际眼科杂志（英文版）

摘　　要：~ AIM： To study the effects of curcumin on the secretion of interleukin （IL） -6 and IL-8 by corneal limbus epithelial cells. METHODS： Human corneal Iimbus epithelial cells were isolated and cultured from donor eyes and irradiated by UVB at different dosages with or without curcumin. MTT test was used for studying the effects of UVB and curcumin on the cell viability. The role of mitogen-activated protein kinase （MAPK） and nuclear factor-kappa B （NF-KB） pathways on the UVB-induced secretion of IL.6 and IL-8 were tested by addition of their inhibitors to the culture with or without UVB-radiation. Levels of various signal pathways, IL-6 and IL-8 in the cells and in the conditioned culture medium were measured by ELISA analysis. RESULTS： UVB at 20 mJ/cm2 or less and curcumin at 20 IJmol/L or less did not affect the cell viability of culturedlimbus epithelial cells （P〉0.05）. UVB irradiation at 10 and 20 mJ/cm2 induced a significant increase of secretion of IL-6 and IL-8 and upregulated NF-KB and phosphorylated MAPK pathways of cultured limbus epithelial cells （P〈0.05）. Various signal pathway inhibitors, including SP600125 （JNK inhibitor）, SB203580 （p38 MAPK inhibitor） and BAY11-7082 （NF-KB inhibitor） significantly decreased the UVB-induced secretion of IL-6 and IL-8 secretion （P〈0.05）. Curcumin at 5-20 pmol/L significantly inhibited UVB- induced secretion of IL-6 and IL-8 by limbus epithelial cells in a dose-dependent manner; while curcumin alone did not affect the secretion of IL-6 and IL-8. The upregulation of NF-KB and MAPK pathways induced by UVB treatment was significantly inhibited by curcumin, suggesting that NF-KB and MAPK pathways are involved in the inhibitory effect of curcumin on UVB-induced production of IL-6 and IL-8.~ AIM： To study the effects of curcumin on the secretion of interleukin （IL） -6 and IL-8 by corneal limbus epithelial cells. METHODS： Human corneal Iimbus epithelial cells were isolated and cultured from donor eyes and irradiated by UVB at different dosages with or without curcumin. MTT test was used for studying the effects of UVB and curcumin on the cell viability. The role of mitogen-activated protein kinase （MAPK） and nuclear factor-kappa B （NF-KB） pathways on the UVB-induced secretion of IL.6 and IL-8 were tested by addition of their inhibitors to the culture with or without UVB-radiation. Levels of various signal pathways, IL-6 and IL-8 in the cells and in the conditioned culture medium were measured by ELISA analysis. RESULTS： UVB at 20 mJ/cm2 or less and curcumin at 20 IJmol/L or less did not affect the cell viability of culturedlimbus epithelial cells （P〉0.05）. UVB irradiation at 10 and 20 mJ/cm2 induced a significant increase of secretion of IL-6 and IL-8 and upregulated NF-KB and phosphorylated MAPK pathways of cultured limbus epithelial cells （P〈0.05）. Various signal pathway inhibitors, including SP600125 （JNK inhibitor）, SB203580 （p38 MAPK inhibitor） and BAY11-7082 （NF-KB inhibitor） significantly decreased the UVB-induced secretion of IL-6 and IL-8 secretion （P〈0.05）. Curcumin at 5-20 pmol/L significantly inhibited UVB- induced secretion of IL-6 and IL-8 by limbus epithelial cells in a dose-dependent manner; while curcumin alone did not affect the secretion of IL-6 and IL-8. The upregulation of NF-KB and MAPK pathways induced by UVB treatment was significantly inhibited by curcumin, suggesting that NF-KB and MAPK pathways are involved in the inhibitory effect of curcumin on UVB-induced production of IL-6 and IL-8.

关 键 词：CURCUMIN ULTRAVIOLET-B INTERLEUKIN-6 INTERLEUKIN-8 comeal limbus epithelial cells PTERYGIUM 

分 类 号：R777.33[医药卫生—眼科]