P因子介导mir-2基因簇原位过表达调节Yki的活性分析  

In Situ Overexpression of mir-2 Enhances Yki Activity in Drosophila Melanogaster

作　　者：李珊珊[1] 林晓惠[1] 吴世安[1] 

机构地区：[1]南开大学生命科学学院,药物化学生物学国家重点实验室,天津300071

出　　处：《南开大学学报（自然科学版）》2017年第3期54-59,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：自然科学基金面上项目(31471319)

摘　　要：Hippo信号通路在动物器官大小发育和肿瘤发生过程中发挥关键性作用.为了发掘新的Hippo信号通路活性调节因子,通过P因子插入介导基因原位过表达,在果蝇表型修饰筛选中鉴定了1株可以显著增强Hippo信号通路下游效应因子Yki活性的果蝇株MS004.反向PCR定位显示,MS004中P因子位于2号染色体左臂蛋白编码基因spitz的内含子区域;进一步的遗传分析确定,MS004果蝇株通过诱导位于spitz内含子区域的mir-2基因簇原位过表达增强Yki的活性.Hippo signaling pathway plays crucial roles for organ size control and tumorigenesis in animal and human being. To uncover novel modulators for Hippo signaling in Drosophila, we performed a modifier screen and identified that MS004 line significantly enhanced the activity of Yki in Drosophila eyes.MS004 harbors an engineered P-element in its genome which could be used to induce gene or gene combination overexpressing in situ. To investigate which gene induced by inserted P-element in enhancing Yki, the P-element of MS004 was determined to be located in the intron region of spitz on 2 L by inverse PCR, and the cluster of mir-2 gene family in this region responds to enhance Yki activity.

关 键 词：mir-2基因簇 Hippo信号通路 反向PCR P因子 

分 类 号：Q78[生物学—分子生物学]