不同功率密度HMME-PDT对致龋模型防龋效果的影响  

The effects of HMME-PDT with different power density on the prevention of dental caries in rat model

作　　者：马莹莹 邹朝晖[2] 李敏[2,3] 张蕾[2,4] 

机构地区：[1]郑州市口腔医院,河南郑州470000 [2]天津医科大学口腔医院,天津300070 [3]天津市第二医院,天津300070 [4]烟台市口腔医院,山东烟台264000

出　　处：《牙体牙髓牙周病学杂志》2017年第6期320-325,共6页Chinese Journal of Conservative Dentistry

摘　　要：目的:观察以血卟啉单甲醚(HMME)为光敏剂的不同功率密度光动力疗法(PDT)对大鼠致龋模型防龋效果的影响。方法:取21 d龄Wistar大鼠56只,并通过口腔接种变异链球菌而建立致龋模型后,将其随机分为7组(n=8),分别为:3个不同功率密度(70、140、280 mw/cm2HMME-PDT)光动力组、单纯激光照射组、单纯光敏剂组、9 m L/L生理盐水(阴性对照)组、2 g/L氟化钠(阳性对照)组。然后按上述分组对各大鼠的上下颌磨牙进行相应处理;每周处理1次,连续4周后处死所有大鼠,用Keyes法记分并评价各组的防龋效果。结果:不同功率密度PDT组和氟化钠组的Keyes记分均明显低于生理盐水组、单纯激光照射组、单纯光敏剂组(P<0.05);Keyes记分结果显示,70 mw/cm2组明显高于氟化钠组(P<0.05),140 mw/cm2组与氟化钠组相近(P>0.05),280 mw/cm2组明显低于氟化钠组(P<0.05)。表现为:随着功率密度的增加,其Keyes记分呈逐渐降低的趋势。结论:HMME-PDT对大鼠龋损有明显的防治作用,其效果与功率密度密切相关。AIM： To evaluate the inhibitive effect of photodynamic therapy（PDT） with different power density on dental caries formation in rats.METHODS：56 Wistar rats （aged 21 days） were infected with S.mutans for the establishment of rat caries mode1s and then divided in to 7 groups（n=8）.As controls, the rat＇＇s teeth were treated with 9 mL/L NS, and 2 g/L NaF respectively.The laser, hematoporyrin monomethyl ether（HMME） and laser plus HMME were respectively used to treat the teeth in the treatment groups, The rats＇＇ teeth were treated once a week for 4 weeks.Then the rats were sacrificed and Keyes count method was used to evaluare the inhibitory effect of PDT on dental caries.RESULTS： The PDT-treated teeth and NaF-treated teeth showed significantly reduced Keyes scores than the negative controls, laser-treated and HMME-treated teeth（P〈0.05）.Keyes scores of 70 mw/cm2 HMME-PDT group was higher than that of NaF-treated teeth （P〈0.05）.Keyes scores of 140 mw/cm2 HMME-PDT group was the same as that of NaF-treated group（P〉0.05）, Keyes scores of 280 mw/cm2 HMME-PDT group was less than that of NaF-treated group （P〈0.05）.Keyes scores reduced with the increase of the laser power dentistry.CONCLUSION： HMME-PDT may inhibite caries development in a laser power density dependent manner.

关 键 词：功率密度 血卟啉单甲醚(HMME) 光动力疗法(PDT) Keyes龋损计分 

分 类 号：R781.1[医药卫生—口腔医学]