恩替诺特对K562人慢性粒细胞白血病细胞株G_0/G_1细胞周期的影响  

Effects of entinostat on G_0/G_1 cell cycle of human chronic myelocytic leukemia cell line K562

作　　者：朴哲[1] 林昌岫[2] 马天泽[1] 

机构地区：[1]延边大学附属医院血液科 [2]延边大学附属医院中心实验室,吉林延吉133000

出　　处：《延边大学医学学报》2017年第1期15-17,共3页Journal of Medical Science Yanbian University

基　　金：吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2015]第45号);延边大学科研项目(2013700-602014001);吉林省卫生计生委课题(2014ZC056)

摘　　要：[目的]探讨恩替诺特对K562人慢性粒细胞白血病细胞株G_0/G_1细胞周期的影响.[方法]采用CCK-8试剂盒观察恩替诺特对体外培养的K562人慢性粒细胞白血病细胞生长抑制的影响,并利用流式细胞仪检测细胞周期.[结果]CCK-8试剂盒观察结果显示,与对照组比较,随着恩替诺特药物浓度的增加及作用时间的延长,K562细胞抑制率显著升高(P<0.05).流式细胞仪分析结果显示,恩替诺特可使K562细胞阻滞于G_0/G_1期,且阻滞细胞的数量与药物浓度呈正相关(P<0.05);恩替诺特作用48h时对细胞周期的阻滞作用最强.[结论]恩替诺特通过阻滞细胞周期于G_0/G_1期而抑制K562细胞增殖.OBJECTIVE To explore the effects of entinostat on G0/G1 cell cycle of human chronic myelocytic leukemia cell line K562.METHODS CCK-8kit was used to observe the effects of entinostat on the proliferation of K562 cells,and flow cytometry was used to detect the cell cycle.RESULTS The results of CCK-8kit showed that the inhibition rate of K562 cells was significantly increased with the increase entinostat concentration and action time as compared with the control group（P〈0.05）.Flow cytometry showed that K562 cells were arrested in G0/G1 phase in a dose dependent manner（P〈 0.05）.The retardation effect on cell cycle was strongest on the time of 48 hafter entinostat exposure.CONCLUSION Entinostat could inhibit the proliferation of K562 cells by arresting cell cycle in G0/G1 phase.

关 键 词：慢性粒细胞白血病 恩替诺特 K562 细胞周期 

分 类 号：R733.72[医药卫生—肿瘤]