载药纳米粒明胶纤维缓释支架对牙周膜细胞MMPs的影响  

Drug-loaded Nanoparticles within Gelatin Scaffolds Inhibits MMPs Expression in Periodontal Ligament Cells

作　　者：王雅冰[1] 苏俭生[1] WANG Ya-bing SU Jian-sheng(Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, Chin)

机构地区：[1]同济大学口腔医学院·同济大学附属口腔医院口腔修复教研室,上海牙组织修复与再生工程技术研究中心,上海200072

出　　处：《口腔颌面外科杂志》2016年第6期385-392,共8页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(81371949、81572114);上海市科学技术委员会项目(13411951201)

摘　　要：目的 :研究载免疫抑制剂SB203580的纳米粒-明胶(Ms-SB203580-gelatin)纤维缓释支架对牙周膜细胞(human periodontal ligament cells,h PDLCs)的基质金属蛋白酶(matrix metalloproteninases,MMPs)表达的影响。方法 :通过透析法制备载药纳米胶束(Micelles-SB203580,Ms-SB203580),并通过电纺法构建载纳米粒明胶支架,检测支架材料的细胞相容性和对牙周膜细胞MMP-2、MMP-13基因和蛋白的表达影响。结果:透射电镜显示载药纳米粒能负载于明胶纤维中;细胞与支架复合后增殖情况良好;实验组MMP-2、MMP-13的表达低于阳性对照组。结论:载药纳米粒明胶纤维缓释支架能明显地抑制炎症因子,可进一步应用于牙槽骨骨组织工程。Objective： The study aimed to evaluate whether the SB203580-1oaded nanoparticles-gelatin （Ms-SB203580- gelatin） scaffolds are related to reduction of matrix metalloproteninases（MMPs） expression in human periodontal ligament cells （hPDLCs）. Methods： The drug-loaded micelles （Micelles-SB203580, Ms-SB203580）was obtained by dialysis bag method and fabricate drug-loaded gelatin scaffolds by electrospining. Cell compatibility and the gene as well as protein ex- pression of MMP-2,MMP-13 were measured. Results： The gelatin scaffolds contain drug-loaded micelles were proved by TEM, The hPDLCs seeded at the scaffolds demonstrated a good biocompatibility. MMP-2 and MMP-13 expression were stain slightly in the experimental group than that of the positive group. Conclusion： The results suggest that Ms- SB203580-gelatin scaffolds have a potential to obviously reduce inflammation and may be effective for alveolar bone repair in the future.

关 键 词：免疫抑制剂 支架 牙周膜细胞 MMPS 

分 类 号：R781.4[医药卫生—口腔医学]