马缨丹提取物对黄胸散白蚁体内酶活性的影响  

Effects of Extracts of Lantana camara (Verbenaceae) Leaves on Cellulose and Detoxifying Enzymes Activities of Reticulitermes flaviceps (Isoptera: Rhinotermitidae)

作　　者：孙骊珠 罗兰[1] 袁忠林[1] Sun Lizhu Luo Lan Yuan Zhonglin(College of Agronomy and Plant Protection, Qingdao Agricultural University Qingdao 266109)

机构地区：[1]青岛农业大学农学与植物保护学院,青岛266109

出　　处：《林业科学》2017年第5期107-115,共9页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(31272106);山东省"泰山学者"建设工程专项

摘　　要：【目的】探究马缨丹氯仿提取物、乙酸乙酯和石油醚萃取物对黄胸散白蚁体内纤维素酶系及解毒酶系活性的影响,初步探讨其作用机制。【方法】采用滤纸片带毒饲喂,测定处理不同时间点其对黄胸散白蚁体内3种纤维素酶[内切-β-1,4-葡萄糖酶(EG)、外切-β-1,4-葡萄糖酶(CBH)、β-葡萄糖苷酶(BG)]和3种解毒酶[羧酸酯酶(Car E)、谷胱甘肽S-转移酶(GSTs)、多功能氧化酶(MFO)]酶活性的影响。【结果】马缨丹氯仿提取物、乙酸乙酯和石油醚萃取物在6~72 h对黄胸散白蚁体内6种酶活性的影响存在差异,主要表现为有一定的抑制作用。对3种纤维素酶活性的影响而言,氯仿提取物对EG酶活性无显著影响,但对CBH和BG酶有显著的抑制作用,尤其对CBH酶抑制作用较强,在72 h时抑制率最大,达17.61%;乙酸乙酯萃取物对3种酶均有抑制作用,对EG和BG酶活性抑制作用较强,在48 h时对EG的抑制率最大,为27.18%,在24 h时对BG酶活性抑制作用最大,为29.28%;石油醚萃取物对CBH酶活性无显著影响,但对其他2种酶抑制作用显著,对EG酶的抑制作用较强,在72 h时对此酶的抑制作用最大,为39.89%。对3种解毒酶活性影响而言,3种提取物对Car E酶的活性影响不大;对GSTs酶的影响主要在12~48 h,其中乙酸乙酯萃取物的抑制作用最强,36 h时抑制率最大,为39.54%;对FMO酶的抑制作用呈波动式变化,其中以12 h、36 h抑制作用较强。【结论】马缨丹氯仿提取物、乙酸乙酯和石油醚萃取物能抑制黄胸散白蚁体内的纤维素酶和解毒酶,扰乱其正常的生理代谢功能。[ Objective ] In order to explore the action mechanisms, the effects of 3 different solvent extracts from Lantana camara （Verbenaceae） leaves on cellulose and detoxification enzyme in the termite Reticulitermes flaviceps （Isoptera： Rhinotermitidae） were studied. [ Method]Termites were fed with filter paper saturated with 3 different solvent extracts of L. camara leaves for different times, and then we measured the enzymes activities of endo-13-1,4-glycanase （EG）, exo- 1,4-glucanase （CBH）, 13-1,4-glucosidase （BG）, carboxylesterase （CarE）, glutathione-S-transferase （GSTs） and mixed-functional oxidase （MFO）. [ Result] The all extracts had to some degree inhibiting effects on the six enzymes activities, however there were differences in the inhibition with extracts of chloroform, ethyl acetate and petroleum ether at 6 -72 h. As for the three cellulose enzymes, the chloroform extract had no significant effect on EG, but had significantly inhibiting effect on CBH and BG, especially on CBH with the highest inhibition rate of 17.61% at 72 h. The ethyl acetate extract also had inhibiting effect on the 3 enzymes activities, with relatively stronger inhibition on EG and BG, and the inhibition rate on EG was 27. 18% at 48 h, and 29.28% at 24 h, respectively. The petroleum ether extract had no significant effect on CBH, but had strongly inhibited on EG and BG, with the highest inhibition rate of 39.89% on EG at 72 h. As for the three detoxification enzymes, the 3 extracts had no significant effect on CarE, but had inhibiting effect on GSTs, which mainly occurred at 12 -48 h. The ethyl acetate extract had the strongest inhibition, with the highest inhibition rate of 39.54% at 36 h. The MFO activities fluctuated after being treated with 3 extracts, and the relatively stronger inhibition on the 3 detoxification enzymes occurred at 12 h and 36 h. [ Conclusion] The extracts of chloroform, ethyl acetate and petroleum ether could decrease the cellulose and detoxification enzymes activities, disrupti

关 键 词：黄胸散白蚁 马缨丹提取物 纤维素酶 解毒酶 杀虫作用机制 

分 类 号：S718.7[农业科学—林学]