新疆两色金鸡菊中原花青素含量测定体系考察  

The Determination of Proanthocyanidins Content in Coreopsis tinctoria Nutt. from Xinjiang in Different Chromogenic Reaction System

作　　者：刘谢英[1] 冯玉洁[1] 冶晓童 姚新成[1,2] 

机构地区：[1]石河子大学药学院,新疆石河子832000 [2]石河子大学新疆特种植物药资源教育部重点实验室,新疆石河子832000

出　　处：《食品研究与开发》2017年第12期119-122,152,共5页Food Research and Development

基　　金：国家自然基金项目(81660641);新疆生产兵团重点领域科技攻关项目(2014BA031);石河子大学高层次人才科研启动项目(RCZX201328)

摘　　要：为准确测定新疆两色金鸡中原花青素含量,选择合适的对照品和测定体系。通过比较香草醛-盐酸、正丁醇-盐酸、铁盐(Fe^(3+))催化法、pH示差法等多种测定体系下,两色金鸡菊提取物和对照品儿茶素或原花青素的连续波长扫描图谱,考察光谱吸收曲线的一致性和λ_(max)峰位值,进而选择适合测定两色金鸡菊中原花青素含量的显色反应体系。结果表明,两色金鸡菊提取物与原花青素对照品在正丁醇-盐酸、Fe^(3+)催化法和pH示差法产生的光谱吸收曲线相似,λ_(max)峰位值更接近。因此,可以用这3种体系快速测定两色金鸡菊中原花青素的含量。结果显示,在特定的测定体系中,选择合适的对照品能准确测定新疆两色金鸡菊中原花青素的含量。To select the appropriate standards and chromogenic reaction system for determination the content of proanthocyanidins in Coreopsis tinctoria flowering tops （CTFT） from Xinjiang, the spectrum of CTFF extract, catechin and proanthocyanidins standards were compared in vanillin-hydrochloric acid, n-butanol-hydrochloric acid, Fe3＋ catalytic chromogenic method and pH differential spectrophotometry reaction system. The spectral absorption curve and the wavelength λmax peak values were investigated for selecting a suitable reaction system to determine the content of proanthocyanidins of CTFT. The spectrum of CTFT extract and proanthocyanidins stan- dard were similar in n-butanol-hydrochloric acid, Fe3＋ ehromogenic method and pH differential spectrophotom- etry reaction system, and the λmax, values were also closer to standard. Therefore, the content of proanthocyanidins in CTFT could be assayed by these three chromogenic reaction system. The content of proanthocyanidins in CTFT could be accurately determined in a particular reaction system and using an appropriate standard.

关 键 词：两色金鸡菊 原花青素 显色反应体系 

分 类 号：TS207.3[轻工技术与工程—食品科学]