猪皮中胶原蛋白的提取与结构鉴定  

Extraction of Pigskin Collagen and Its Structural Identification

作　　者：纪倩[1] 宿丹丹 应慧妍 陈敬华[1] JI Qian SU Dan-dan YING Hui-yan CHEN Jing-hua(Laboratory of Pharmaceutics and Pharmaceutical Materials Science, Jiangnan University, Wuxi 214122, Jiangsu, China)

机构地区：[1]江南大学药剂学与药剂材料学研究室,江苏无锡214122

出　　处：《食品研究与开发》2017年第13期44-49,共6页Food Research and Development

基　　金：江苏省产学研联合创新资金项目(BY2014023-17)

摘　　要：以猪皮为原料,使用酶法提取胶原蛋白,对提取条件和过程进行筛选和优化,并对胶原产物的结构性质进行表征。结果表明,胶原蛋白产物的提取率为65.33%;纯度为87.38%;热变性温度在118.79℃;紫外最大吸收波长为203 nm;相对分子质量为3.63×10~5 g/mol;红外光谱和圆二色谱结果显示,产物具有典型的胶原蛋白吸收峰,说明提取出的胶原蛋白三螺旋结构保存完整;十二烷基硫酸钠聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate polyacrylamide gel electrophoresis,SDS-PAGE)结果显示所得胶原产物主要由3种亚基组分组成。The research used pigskin as raw material, which was treated by enzymes, to extract collagen. To confirm and optimize the best extraction condition and process, the structural properties of collagen product were characterized. The experiment results showed that the extraction yield of collagen product was 65.33%and the purity was 87.38 %. The thermal denaturation temperature was 118.79 ℃. The maximum UV absorption wavelength was 203 nm. The relative molecular mass was 3.63 ×10^5 g/mol. Infrared spectrum and round two chromatographic experiments showed that the product had a typical collagen absorption peak, indicating the three helical structure of collagen extracted was intact; SDS-PAGE gel electrophoresis test showed that the product was mainly composed of three sub components.

关 键 词：猪皮 胶原蛋白 提取 纯化 结构鉴定 

分 类 号：TS251.92[轻工技术与工程—农产品加工及贮藏工程]