小菜蛾传染性软腐病病毒的全基因组序列及其进化分析  

Phylogenetics analysis of the complete genome of Diamondback moth iflavirus

作　　者：陈大嵩 戴建青 韩诗畴 

机构地区：[1]广东省生物资源应用研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州510260

出　　处：《环境昆虫学报》2017年第3期618-631,共14页Journal of Environmental Entomology

基　　金：国家自然科学基金(31371932);广东省科学院青年科学研究基金(qnjj201602);广东省科学院科技发展专项(2017GDASCX-0107)

摘　　要：传染性软腐病病毒(Iflavirus)属于小核糖核酸病毒目,是一类单股正链RNA病毒,其家族成员正在不断扩大。Iflavirus的寄主包括鳞翅目、膜翅目和半翅目的昆虫以及瓦螨,因其对一些重要的农业害虫产生致病性,因此是重要的潜在生防病毒种类之一。本研究利用小菜蛾转录组测序数据拼接出一条Iflavirus病毒基因组序列。通过查找ORF和序列相似性比对发现此基因组可编码一条完整的病毒多聚蛋白并与其他Iflavirus病毒同源,因此将这一新病毒命名为小菜蛾传染性软腐病病毒(Diamondback moth iflavirus),英文简称DBMIV。通过多聚蛋白序列与RNA聚合酶序列构建的进化树表明DBMIV与PNV、EOPLV和SEIV2进化关系最近。最后,利用RT-PCR扩增出DBMIV的外壳蛋白区域验证了新命名病毒在田间小菜蛾种群的感染。DBMIV的鉴定与命名为利用高通量测序数据鉴定病毒新种提供参考,为利用病毒生防重要蔬菜害虫小菜蛾提供研究思路。The growing number of Iflaviruses are positive strind RNA viruses, which belong to Picornavirales. The hosts of Iflaviruses include Lepidoptera, Hymenoptera, Hemiptera and Varroa destructor. Iflaviruses are pathogenic to some main agricultural pests and are the potential viruses that could be used in biological control. In this study we assembled a complete Iflavirus genome from the transcriptome data of diamond moth. We find an ORF in this genome which can encode a complete polyprotein homologue to other Iflavirus genomes. Therefore,we nominate this new virus as Diamondback moth iflavirus,and the abbreviation is DBMIV. The phylogenetic analysis based on the polyprotein and RNA polymerase sequences showed that the DBMIV was evolutionally closed to PNV,EOPLV and SEIV2. Eventually,DBMIV was verified to infect the DBM in the field by RT-PCR amplification of the coat protein region. The identification of the DBMIV provided a referential scheme of virus species identification from high-through sequencing data,and offered a new research approach of virus control to the diamond moth.

关 键 词：小菜蛾 传染性软腐病病毒 基因组 系统进化 

分 类 号：Q968.1[生物学—昆虫学] S433.4[农业科学—农业昆虫与害虫防治]