紫草萘醌衍生物联合放射线照射抑制人鼻咽癌细胞增殖和诱导凋亡  

Sensibilization and apoptosis induction of radix arnebiae naphthoquinone derivative combined with radiotherapy on human nasopharyngeal carcinoma cell lines

作　　者：苏怡[1] 谢景华[1] 孟庆翔[1] 何龙[1] 曾国庆[1] 张改霞[1] 

机构地区：[1]广州市第一人民医院耳鼻咽喉头颈外科,广东广州510180

出　　处：《中国耳鼻咽喉颅底外科杂志》2017年第3期207-211,216,共6页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：广州市医药卫生科技项目(20151A010015)

摘　　要：目的探讨紫草萘醌衍生物(SYUNZ-4)[3,11-bis(2-hydroxyethansulfanyl)-6-isohexeny lnaphthazarin]联合放射治疗对鼻咽癌(nasopharyngeal carcinoma,NPC)细胞生长的协同抑制作用及放疗增敏机制。方法人NPC高分化鳞状细胞癌细胞株CNE1和低分化鳞状细胞癌细胞株CNE2随机分为空白对照组、单纯SYUNZ-4组、单纯放射治疗组及SYUNZ-4联合放射线照射组共4组。单纯放射治疗组仅给予剂量4 Gy放射线照射,单纯SYUNZ-4组仅给予最大非细胞毒性剂量的SYUNZ-4液培养,SYUNZ-4联合放射线照射组在给予4 Gy的放射线照射后,立即给予最大非细胞毒性剂量的SYUNZ-4液培养;然后应用流式细胞术检测SYUNZ-4诱导CNE1和CNE2细胞凋亡的作用及其对细胞周期的影响。结果 SYUNZ-4对CNE1和CNE2细胞均具有增殖抑制作用,且其作用呈浓度依赖性。与空白对照组相比,单纯放射线照射组及联合治疗组细胞增殖均受到抑制(P<0.05),且前者G2/M期细胞比例明显增加(P<0.05),后者S期及G2/M期细胞比例均显著增加(P<0.05)。与单纯放疗组相比,联合干预使CNE1和CNE2细胞增殖受到更明显地抑制(P<0.01)。与对照组相比,放射线照射导致细胞凋亡率明显增加(P<0.05);而联合治疗组与单纯放疗组和空白对照组相比,联合干预使CNE1和CNE2细胞凋亡率明显增加,组间差异均具有统计学意义(P<0.05)。结论紫草萘醌衍生物SYUNZ-4联合放射线照射能显著抑制人NPC细胞的增殖,并导致NPC细胞周期阻滞和诱导凋亡。Objective To investigate the mechanism of synergistic repression and sensibilization of radix arnebiae naphthoquinone derivative [ 3, 11-his （ 2-hydroxyethansulfany] ） 45-isohexenyl naphthazarin, SYUNZ4 ] combined with radiotherapy on human nasopharyngea] carcinoma （NPC） cell lines. Methods Human NPC well-differentiated squamous cell carcinoma cell line CNE1 and poorly differentiated squamous cell carcinoma cell line CNE2 were randomly divided into four groups ： blank control group, SYUNZ-4 group, radiotherapy group, combination of SYUNZ4 with radiotherapy group. The radiation dose was 4Gy and cell lines of the combined group were cultured with the maximal non-toxic dose of SYUNZ- 4 fight after finishing radiotherapy. After that, apoptosis induction and effect on cell cycle distribution were studied by flow eytometry. Results The proliferations of CNE1 and CNE2 were significantly inhibited by SYUNZ4 in a concentration- dependent manner. Compared with the blank control group, the cell proliferations were remarkably inhibited in both the radiotherapy group and the combined group （ both P 〈 0.05 ）, the proportion of G2/M cells was increased significantly （P 〈0.05） in the radiotherapy group and that of S and G2/M cells was increased significantly in the combined group （P 〈 0.05 ）. Compared with the radiotherapy group, cell proliferations of CNE1 and CNE2 were more significantly inhibited by combined intervention（ both P 〈0.01 ）. Compared with the control group, radiation exposure led to a significant increase in apoptosis （P 〈0.05）. Moreover, the apoptotic rates of CNE1 and CNE2 cells in the combined group were significantly increased compared with the radiotherapy group （ P 〈 0. 05 ）. Conclusion SYUNZ4 combined with radiotherapy can significantly inhibit the proliferation of human NPC cells, which results in cell cycle arrest and apoptosis induction.

关 键 词：紫草萘醌衍生物 鼻咽癌 放疗增敏 细胞周期阻滞 细胞凋亡 

分 类 号：R739.63[医药卫生—肿瘤]