2013～2014年我国输入性B3基因型麻疹病毒的基因特征分析  

Genetic Characteristic Analysis of Imported B3 Genotype Measles Virus in Mainland of China during 2013～2014

作　　者：王慧玲[1] 王淑蕾[2] 任丽[1] 毛乃颖[1] 张燕[1] 许文波[1,3] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,卫生部医学病毒学和病毒病重点实验室,世界卫生组织西太平洋区麻疹参比实验室,北京102206 [2]烟台市疾病预防控制中心,烟台264003 [3]安徽理工大学医学院,淮南232001

出　　处：《病毒学报》2017年第4期577-582,共6页Chinese Journal of Virology

基　　金：国家自然科学基金课题(项目号:81371791),题目:麻疹病毒血凝素蛋白分子进化抗原变异及疫苗保护效果的影响~~

摘　　要：研究2013~2014年中国大陆分离到的输入性B3基因型麻疹野毒株的N蛋白羧基端核苷酸和氨基酸特征。应用MEGA6.0软件对2013~2014年输入我国的B3基因型麻疹病毒、GenBank下载的WHO参考株、2013~2014年全球流行的B3基因型麻疹病毒代表株和中国疫苗株泸191(S191)构建基于N蛋白COOH端450个核苷酸片段序列的亲缘性关系树、分析其核苷酸和氨基酸差异。13株B3基因型中国分离株间的核苷酸序列和氨基酸序列同源性分别为99.7%~100%和100%;与B3基因型WHO参考株的核苷酸和氨基酸同源性分别为96.6%~96.8%和95.3%~97.3%;与2013~2014年流行的B3基因型的麻疹野病毒代表株的核苷酸和氨基酸同源性分别在90.0%~100%和87.9%~100%;与S191的核苷酸和氨基酸同源性分别为93.3%~93.5%和87.2%。本研究对积累我国麻疹病毒分子流行病学基线数据具有很重要的意义,随着我国进入麻疹消除加速阶段,将会监测到更多的非本土基因型的输入,需要进一步加强病毒学监测,防止输入性麻疹野病毒在我国扩散和传播。To study the genetic characterization and amino acid mutation of 450 nucleotide sequence in the carhoxyl terminus of measles virus nucleoprotein of imported B3 genotypes measles virus in mainland of China during 201342014. The phylogenetic tree based on the 450 nucleotides of carboxyl terminus of N protein was constructed and the homology similarity was analyzed. The Chinese viruses were clustered within the same genotype group with WHO B3 genotype reference strain. The nucleotide sequence and predicted amino acid homologies of 13 B3 genotype viruses were 99.7-100% and 100%, respectively, compared with 2013-2014 B3 genotype representative strains and Chinese vaccine S-191, the homology of nucleotide sequence were 90.0 - 100 % and 93.3-93.5 %, the homology of amino acid sequences were 86.7-89.3% and 87.2%, respectively. This paper is important to accumulate baseline data of genetic variation of measles viruses circulating in China. In the stage of the measles elimination, more non-endemic measles viruses are expected to be imported to the country, therefore it is necessary to further strengthen the surveillance of the measles virus circulating in China to provide the scientific evidence of interruption of imported measles viruses spread.

关 键 词：麻疹病毒 病毒学监测 B3基因型 亲缘性分析 

分 类 号：R373.11[医药卫生—病原生物学]