5-氮杂胞苷对多发性骨髓瘤MM1R及RPMI8226细胞增殖的影响  

THE EFFECT OF 5-AZA-CYTIDINE ON THE PROLIFERATION OF MULTIPLE MYELOMA CELL LINE MM1R AND RPMI8226

作　　者：樊馥荔 王伟[1] 李广伦[1] 吴颖[1] 鞠小妍[1] 王颜刚[2] 

机构地区：[1]青岛大学附属医院血液科,山东青岛266003 [2]青岛大学附属医院内分泌科,山东青岛266003

出　　处：《青岛大学医学院学报》2017年第2期150-152,156,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨甲基化抑制剂5-氮杂胞苷对人多发性骨髓瘤耐药株MM1R及普通株RPMI8226细胞增殖的影响。方法培养人多发性骨髓瘤细胞株MM1R及RPMI8226至对数生长期,验证MM1R耐药性。加入不同浓度的5-氮杂胞苷共培养,然后用CCK8法测定不同时间MM1R及RPMI8226的增殖活性,采用酶联免疫吸附测定(ELISA)法检测两种细胞的γ干扰素(IFN-γ)表达水平。结果在作用相同时间时,随着5-氮杂胞苷浓度增高,MM1R和RPMI8226的增殖活性均降低(F=78.68、63.12,P<0.05)。在同一浓度5-氮杂胞苷作用下,随着时间的延长,MM1R和RPMI8226的增殖活性降低(F=112.12、99.87,P<0.05)。同一浓度5-氮杂胞苷作用相同时间,MM1R的细胞增殖抑制率较RPMI8226高。不同浓度的5-氮杂胞苷处理细胞48h,两种细胞的IFN-γ表达水平均随5-氮杂胞苷浓度升高而增高(F=46.78、58.67,P<0.05)。结论 5-氮杂胞苷对人多发性骨髓瘤MM1R及RPMI8226细胞增殖均有抑制作用,并且对MM1R的抑制作用强于对RPMI8226的抑制作用。同时,5-氮杂胞苷有促进IFN-γ分泌的作用。Objective To investigate the effect of 5-aza-cytidine on the proliferation of multiple myeloma cell line MM1 R and RPMI8226. Methods Human multiple myeloma cell line MM1 Rand RPMI8226were cultured to logarithmic growth phase and MM1 Rresistance was verified.Different concentrations of 5-aza-cytidine were added to co-culture with MM1 Rand RPMI8226.The proliferation activity of MM1 Rand RPMI8226at different time was detected using CCK8,the expression of IFN-γin MM1 R and RPMI8226 was measured applying ELISA. Results At the same action time,the proliferative activity of MM1 Rand RPMI8226decreased with the increase of concentration of 5-aza-cytidine（F=78.68,63.12;P〈0.05）.Under the same concentration of 5-aza-cytidine,the proliferation of MM1 Rand RPMI8226decreased along with the time extending（F=112.12,99.87;P〈0.05）.When the action time and the concentration of 5-aza-cytidine were the same,the cell proliferation and inhibition rate（CPIR）of MM1 Rwas higher than that of RPMI8226.When these two cells were treated with different concentrations of 5-aza-cytidine,the IFN-γexpression in the two cells increased with the increase of 5-aza-cytidine concentration（F=46.78,58.67;P〈0.05）. Conclusion The 5-aza-cytidine has inhibition action on proliferation of MM1 Rand RPMI8226cells in human multiple myeloma,and the inhibitory effect on MM1 Rwas stronger than that on RPMI8226,and at the same time,5-aza-cytidine has a role in promoting the secretion of IFN-γ.

关 键 词：胞苷 多发性骨髓瘤 细胞增殖 干扰素Γ 

分 类 号：R733.3[医药卫生—肿瘤]