p73和p51在胃癌中的改变及意义  

Alteration of p73 and p51 genes and its significance in human gastric carcinogenesis

作　　者：黄东胜[1] 谢海洋[1] 郑树森[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2002年第4期245-249,共5页Journal of Zhejiang University(Medical Sciences)

摘　　要：目的 :探讨 p53基因家族新成员 p73和 p51基因的两个转录本 p51A、p51B基因转录水平与胃癌发生发展的关系。方法 :采用逆转录多聚酶链反应技术 ( RT- PCR) ,检测 32例胃癌组织及邻近正常胃粘膜组织 p73、p51A及 p51B基因转录水平。结果 :32例胃癌组织有 17例 p73表达阳性 ,相对应的正常胃组织仅有 2例表达阳性 ,两者具有显著性差异 ( P<0 .0 1) ,胃癌组织的 p 73阳性表达率与胃癌的 TNM分期密切相关 ( P<0 .0 5) ;p 51A、p51B基因在 32例标本的胃癌组织和相对应的正常胃组织呈低水平表达 ,其中胃癌组织中 p51A表达量明显高于正常胃组织 ( P<0 .0 5) ,与细胞分化程度无关 ( P>0 .0 5) ,与 TN M分期也无关 ( P>0 .0 5) ,而 p51B在胃癌组织和正常胃粘膜组织表达量无明显差异 ( P>0 .0 5)。结论 :胃癌组织中存在 p73、p51A基因的过度转录表达 ,p73、p51A的过表达与胃癌发生发展有关。Objective: To evaluate the relationship between the expression of p51 , p73 and the oncogenesis and development of human gastric carcinoma. Methods: The expressions of p73 mRNA were detected both in 32 human gastric carcinoma tissues and adjacent normal gastric tissues by reverse transcriptase polymerase chain reaction (RT PCR). Results: Overexpressions of p73 mRNA were found in 17/32 gastric carcinoma tissues,in 2/32 adjacent normal gastric tissues. The positive expression rate of p73 mRNA in gastric carcinoma tissues was significantly higher than that in adjacent normal gastric tissues ( P <0.01). However,a significant correlation was found between the positive expression rate of p73 mRNA in gastric carcinoma tissues and the TNM staging( P <0.05). The low expressions of p51A mRNA and p51B were found in all gastric carcinoma tissues and adjacent normal gastric tissues. the expressions of p51A in gastric carcinoma tissues were much higher than adjacent normal gastric tissues( P <0.05). The expression of p51B is no significant correlation was observed between gastric carcinoma tissues and adjacent normal gastric tissues. Conclusion: The results suggest that there is an overexpression of p73 and p51A mRNA in gastric cancer tissues, and their expressions is relationship with oncogenesis and development of gastric carcinoma.

关 键 词：胃癌 胃肿瘤 P53基因 P73基因 P51基因 RT-PCR 

分 类 号：R735.2[医药卫生—肿瘤]