紫杉醇诱导的凋亡细胞微量元素含量改变的同步辐射研究  

Alteration of the content of minor elements in apoptotic cells induced by anti-cancer drugs with synchronous radiation

作　　者：陈丽荣[1] 郑树[1] 陈于法[1] 张苏展[1] 黄宇营[2] 

机构地区：[1]浙江大学医学院肿瘤研究所,浙江杭州310009 [2]中国科学院高能物理研究所,北京100085

出　　处：《浙江大学学报（医学版）》2002年第4期265-268,272,共5页Journal of Zhejiang University(Medical Sciences)

摘　　要：目的 :探讨细胞凋亡发生过程中是否存在微量元素含量改变并具有生物学意义。方法 :应用同步辐射 X射线荧光检测技术对抗癌药物诱导的乳癌凋亡细胞的微量元素含量进行定量分析。结果 :在凋亡细胞和对照组中均可检测到 7种微量元素包括 Ti,Cr,Fe,Cu,Zn,Ga和 Ge和 5种宏量元素包括 P,S,Ca,Cl和 K。在紫杉醇和秋水仙碱处理 4 8h组凋亡细胞微量元素 Zn和宏量元素 P含量明显升高 ,与对照组比较差异有显著性意义 ( P<0 .0 5)。而微量元素 Fe在紫杉醇处理 4 8h组显著下降 ,与对照组比较亦有显著性意义 ( P<0 .0 5)。抗癌药鬼臼乙叉甙和放线菌酮诱导的凋亡细胞其微量元素含量改变趋势与紫杉醇相似。结论 :微量元素 Zn和 Fe以及宏量元素 P可能参与抗癌药物诱导的细胞凋亡。Objective: To explore the change of content of minor elements and its biological implication in apoptotic processes. Methods: The content of minor elements of apoptotic cells from breast cancer induced by anti cancer drugs was quantitatively analysed with synchronous radiation X ray fluorescence. Results: Seven kinds of minor elements including Ti,Cr,Fe,Cu,Zn,Ga and Ge as well as five major elements P,S,Ca,Cl and K were detected in the apoptotic and control cells. The content of elements Zn and P in apoptotic cells after 48 h treatment with taxol and colchicine was significantly increased higher than that in the control cells ( P <0.05).The content of ele ment Fe in 48 h treatment with taxol was significantly decreased lower than that in the control cells( P <0.05).The same trend of change of elements was observed during the process of apoptotic cells death induced with VP 16 or cycloheximide. Conclusion: This results suggest that the elements Zn, Fe and P should be involved in apoptosis induced by anti cancer drugs.

关 键 词：紫杉醇 诱导 凋亡细胞 微量元素 含量 同步辐射 脱噬作用 乳腺肿瘤 病理学 

分 类 号：R730.231[医药卫生—肿瘤]