阿魏酸对兔耳缺血性慢性创面治疗的影响  

Therapeutical effect of ferulic acid on rabbit ear chronic ischemic wounds

作　　者：余震[1] 余州[2] 雷鸣[2] 石定[2] 李学拥[1] 李靖[1] 彭湃[2] 

机构地区：[1]第四军医大学唐都医院烧伤整形科,西安710032 [2]第四军医大学西京医院整形科

出　　处：《中华医学美学美容杂志》2017年第3期190-194,共5页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：国家自然科学基金（81272118,30973752）

摘　　要：目的 探讨阿魏酸对兔耳缺血性慢性创面的促愈合作用及可能机制.方法 选取40只雄性新西兰大耳白兔,建立兔耳缺血性慢性创面模型后,按对创面处理随机分为凡士林组（凡士林涂抹）、缺血对照组（用0.9％氯化钠注射液冲洗）、5％阿魏酸组（5％阿魏酸涂抹）、重组牛碱性成纤维细胞生长因子（rb-bFGF）组（用rb-bFGF喷涂）;大体观察各组兔耳创面愈合情况,比较创面愈合速度.免疫组织化学及聚合酶链式反应（PCR）检测创面愈合时间及组织中低氧诱导因子-1α（HIF-1α）、血管内皮生长因子（VEGF）及CD31的表达.结果 5％阿魏酸组的愈合时间（16.2±0.4）d与rb-bFGF组（15.92±0.13）d相近,较缺血对照组（18.13±0.33）d及凡士林组（17.2±0.4）d快（P＜0.05）;5％阿魏酸组中VEGF和HIF-1α表达及CD31标记血管数与rb-bFGF组接近,明显高于缺血对照组及凡士林组（P＜0.05）.结论 阿魏酸能通过提高VEGF及HIF-1α等与新生血管密切相关的蛋白表达而促进创面血管生成,进而促进慢性创面愈合.Objective To study whether ferulic acid can promote healing on chronic ischemic wounds and its possible mechanisms.Methods 40 male New Zealand white rabbits were randomly divided into 4 groups：vaseline group,ischemic control group,5% ferulic acid group and recombinant bovine basic fibroblast growth factor for external use （rb-bFGF） group.Gross wounds were carefully observed and HE staining was used to observe the wound healing and immumohistochemical staining to observe the expression of the VEGF and CD31.The RNA was extracted to detect the expression of VEGF and HIF-1a by real-time PCR.Results The general observation and the HE staining of each specimen 11 days after operation all indicated that the duration of wound healing of the 5 % ferulic acid group was similar to that of the rb-bFGF group and markedly shorter than the ischemic control group and the vaseline smear group.The result of the immunohistochemical staining indicated that the content of the VEGF and CD31 expression of the 5 % ferulic acid groups and the rb-bFGF group made lit tle difference,but there was markedly less VEGF and CD31 in ischemic control group and the vaseline smear group.The result of the PCR showed that expression level of VEGF and HIF-1α in the 5 % fer ulic acid group was similar to that in the rb bFGF group and the vaseline smear group,but was obviously more than that of the ischemic control group and the vaseline smear group （P 〈 0.05）.Conclusions Ferulic acid can promote angiogenesis by increasing VEGF and HIF-1α which are closely related to angiogenesis and then promote the healing of chronic wounds.

关 键 词：阿魏酸 兔耳 慢性创面 

分 类 号：R641[医药卫生—外科学]