南极真菌Penicillium sp. S-2-10次级代谢产物的研究  

Research of the secondary metabolites from Antarctic-derived fungus Penicillium sp. S-2-10

作　　者：周玥[1,2] 刘小宇[1,2] 于豪冰[1,2] 卢小玲[1,2] 焦炳华[1,2] ZHOU Yue LIU Xiao-yu YU Hao-bing LU Xiao-ling JIAO Bing-hua(Marine Biopharmaceutical Institute, Second Military Medical University, Shanghai 200433, China Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China)

机构地区：[1]第二军医大学海洋生物医药研究中心,上海200433 [2]第二军医大学基础部生物化学与分子生物学教研室,上海200433

出　　处：《中国海洋药物》2017年第3期18-22,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(41306197;41606173);广东省海洋局公益项目(海洋天然产物化合物库GD2012-D01-001)资助

摘　　要：目的对来源于南极海域海底沉积物真菌Penicilliumsp.S-2-10的次级代谢产物进行研究。方法对菌株进行规模发酵,并对所得发酵液的乙酸乙酯萃取浸膏进行系统分离纯化,据化学指征和生物学活性两方面特点,采用Sephadex LH-20凝胶色谱、正相硅胶柱色谱、反相ODS柱色谱、高效液相柱层析等色谱分析方法,通过核磁(~1 H-NMR和^(13) C-NMR等)及质谱(MS)等波谱分析方法,并与相关文献比较,鉴定化合物结构。结果分离得到7个单体化合物,经鉴定化合物1~7分别为benzoic acid,2-(2,6-dihydroxybenzoyl)-3-hydroxy-5-(hydroxymethyl)-methyl,methyl ester(1),chrysophanol(2),volemolide(3),ergosta-6,8(14),22-trien-3-ol(4),2-aminophenoxazin-3-one(5),2-benzothiazolol(6),benzoic acid(7)。结论化合物3,4为首次从Penicillium属真菌中获得。Objective To study the secondary metabolites of the fungus Penicilliumsp.S-2-10,derived from the Antarctic seabed sediments.Methods The ethyl acetate extract of the fermented liquid extract was purified systematically combining chemical characteristics and bioassays after large scale fermentation,using Sephadex LH-20 gel chromatography,normal phase silica gel column chromatography,reversed phase ODS column chromatography and high performance liquid chromatographic analysis methods.The structures of the compounds were identified on the basis of nuclear magnetic resonance（~1 H and ^（13） C NMR）,mass spectrometry（MS）spectrum and the related literature.Results Compounds 1~7were identified as Benzoic acid,2-（2,6-dihydroxybenzoyl）-3-hydroxy-5-（hydroxymethyl）-methyl,methyl ester（1）,chrysophanol（2）,volemolide（3）,ergosta-6,8（14）,22-trien-3-ol（4）,2-aminophenoxazin-3-one（5）,2-benzothiazolol（6）,benzoic acid（7）.Conclusion Compounds 3 and 4 were firstly isolated from the fungus Penicilliumsp.

关 键 词：南极真菌 青霉属 次级代谢产物 

分 类 号：R914[医药卫生—药物化学]