海洋来源真菌Cochliobolus lunatus产抗污损活性大环内酯化合物zeaenol的发酵优化  

Fermentation optimization for the marine-derived fungus Cochliobolus lunatus to produce antifouling resorcylic acid lactone zeaenol

作　　者：孙继红[1] 张雪晴[1] 杨凯琳[1] 王长云[1,2] 邵长伦[1,2] SUN Ji-hong ZHANG Xue-qing YANG Kai-lin WANG Chang-yun SHAO Chang-lun(Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003,China Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]青岛海洋科学与技术国家实验室,海洋药物与生物制品功能实验室,山东青岛266200

出　　处：《中国海洋药物》2017年第3期29-33,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(41322037);山东省自然科学基金项目(JQ201510);国家海洋局海洋公益性行业科研专项(201405038)资助

摘　　要：目的优化海洋来源真菌Cochliobolus lunatus(TA26-46)的发酵条件,以期提高抗污损活性大环内酯zeaenol的产量。方法运用单因素试验设计和正交试验设计,对该菌株产zeaenol的氮源、前体、盐度和培养基中不同离子等发酵条件进行优化。结果结果表明:最佳优化发酵条件为可溶性淀粉10g·L^(-1),氮源为硝酸钠5g·L^(-1),乙酸钠浓度为5g·L^(-1),盐度为1%。结论在最佳发酵条件下,zeaenol产量可达155.4mg·L^(-1),比优化前提高了5倍。Objective To optimize the fermentation conditions of the marine-derived fungus Cochliobolus lunatus（TA26-46）to increase the yield of the antifouling resorcylic acid lactone zeaenol.Methods The single-factor experiments and orthogonal experiment were designed to investigate the fermentation conditions,including nitrogen source,sodium acetate,salinity and effect of different ions in culture medium.Results The results indicated that the optimized fermentation condition included sodium nitrate（10g·L^-1）,Sodium nitrate（5g·L^-1）,sodium acetate（5g·L^-1）,1% of salinity.Conclusion The yield of zeaenol was improved up to 155.4mg·L^-1,which was five-fold improved.

关 键 词：海洋来源真菌 COCHLIOBOLUS lunatus 大环内酯 发酵条件 优化 

分 类 号：R931[医药卫生—生药学]