不同糖油比例高脂饲料建立高脂血症大鼠模型的比较  

A Comparison on Hyperlipidemia Rat Models Induced by High-fat Diet with Different Proportions of Sugar and Oil

作　　者：覃辉艳[1] 王绍龙[1] 温平镜 赵鹏[1] 李彬[1] 张洁宏[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028

出　　处：《实验动物与比较医学》2017年第3期209-213,共5页Laboratory Animal and Comparative Medicine

基　　金：广西科技基础条件平台建设项目(13-29-04)

摘　　要：目的探讨不同糖油比例高脂饲料建立高脂血症大鼠模型的效果。方法将40只大鼠分为空白对照组和3个高脂饲料(Ⅰ、Ⅱ和Ⅲ)组,每组10只,分别喂饲全价饲料和3种不同糖油比例的高脂饲料,共6周,每周定时称量体质量和检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)。结果 3种配方的高脂饲料对大鼠体质量增长均无明显影响(P>0.05)。各配方组每周的大鼠血清TC含量、LDL-C含量及TC/HDL-C比值均高于空白对照组(P<0.05或P<0.01)。高脂饲料Ⅱ组每周的大鼠血清TG含量均高于空白对照组(P<0.01或P<0.05),高脂饲料Ⅰ组大鼠第1-4周的血清TC含量高于空白对照组(P<0.01或P<0.05),高脂饲料Ⅲ组大鼠血清TG含量除第2周高于空白对照组外其余各周与空白对照组均无统计学差异(P>0.05)。结论高脂饲料Ⅰ和高脂饲料Ⅱ均能快速稳定建立混合型高脂血症大鼠模型,高脂饲料Ⅱ效果更优;高脂饲料Ⅲ仅适用于建立高胆固醇血症大鼠模型。Objective To compare hyperlipidemia rat models induced by high-fat diet with different proportions of sugar and oil. Methods Fourty rats were divided into normal control group and three high-fat diet （I, II and III） groups with 10 rats per each. The normal control group was fed with normal diet, and the other three groups were fed with high-fat diet with different proportions of sugar and oil for 6 weeks respectively. The body weight and the total cholesterol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （HDL-C） and low density lipoprotein cholesterol （LDL-C） in serum were detected weekly. Results No significant difference were observed on body weight among different groups. The TC, LDL-C and TC/HDL-C ratios in all high-fat diet groups were higher than those of normal control group at each time point. The TG in high-fat diet II group was higher than normal control group at each time point, but was only higher at the first 4 weeks in high-fat diet I group, and no significant difference in high-fat diet III group except at second week. Conclusion Both high-fat diet I and II can induce combined hyperlipidemia rat models rapidly and steady, and high-fat diet II is more superior. High-fat diet III may be applied to induce hypercholesterolemia in rat.

关 键 词：高脂饲料 糖和油 高脂血症 大鼠 

分 类 号：R-332[医药卫生] R589.2