KLF8对胶质瘤细胞株U87体外侵袭能力的影响  

Influence of KLF8 on in vitro invasion ability of glioma cell line U87

作　　者：耿飞[1] 孙涛[1] 卢癸凤 

机构地区：[1]遵义医学院生理学教研室,贵州遵义563000 [2]遵义医学院病理生理学教研室,贵州遵义563000

出　　处：《现代医药卫生》2017年第13期1933-1935,共3页Journal of Modern Medicine & Health

基　　金：贵州省卫生和计划生育委员会科学技术基金资助项目(gzwjkj2016-1-009)

摘　　要：目的探讨锌指样因子8(KLF8)对胶质瘤细胞株U87体外侵袭能力的影响。方法瞬时转染针对KLF8的小干扰RNA(siRNA),采用荧光定量聚合酶链反应及蛋白质印迹进行干扰后的效率验证,应用Transwell小室侵袭实验检测U87细胞体外侵袭能力的变化。结果转染KLF8的siRNA 48 h后U87细胞中KLF8 mRNA、蛋白水平均明显下调,体外侵袭能力明显降低。结论 KLF8可促进胶质瘤细胞侵袭,提示其在胶质瘤的侵袭、转移中发挥着重要作用。Objective To investigate the effect of KLF8 on invasion ability ofglioma cellline U87 in vitro. Methods Small interfering RNA （siRNA） aiming at KLF8 was performed by the transient transfection. Fluorescence quantitative PCR and Western blot were used to verify the efficiency after interference. The Transwell chamber invasion test was used to detect the in vitro invasion ability of U87 cells. Results The KLF8 mRNA and protein level were significantly down-regulated in U87 cells at 48 h after transfection of KLF8 siRNA. The in vitro invasion ability was obviously decreased. Conclusion KLF8 can promote glioma cell invasion, indicating that KLF8 plays an important effect in the invasion and migration of glioma.

关 键 词：Krüppel样转录因子 神经胶质瘤 肿瘤浸润 RNA 小分子干扰 

分 类 号：R730.43[医药卫生—肿瘤]