建立大鼠钳夹伤脊髓损伤模型的学习曲线分析  

Learning curve analysis of establishing rat models of spinal cord injury by clip compression

作　　者：陈华[1] 程惠[2] 冷明昊[2] 潘建海[2] 何祥春[2] 张一鸣[2] 卢山[2] 张良[2] 

机构地区：[1]湖北医药学院附属随州医院皮肤科,湖北随州441300 [2]湖北医药学院附属随州医院骨二科,湖北随州441300

出　　处：《右江民族医学院学报》2017年第3期186-189,共4页Journal of Youjiang Medical University for Nationalities

基　　金：湖北省卫生计生科研基金资助(WJ2015MB228)

摘　　要：目的分析大鼠脊髓钳夹伤模型建立的学习曲线。方法将制作大鼠钳夹伤脊髓损伤模型最初的30例资料分为3个阶段(各10例),对手术时间、出血量、假手术组脊髓损伤情况、术后感染、脊髓损伤的BBB运动功能评分、运动诱发电位N1峰(MEP-N1峰)潜伏期时间进行分析,并行病理学HE染色。结果第三阶段操作在手术时间、出血量、假手术脊髓损伤情况、术后感染、BBB评分较第一阶段明显减少,MEP-N1峰潜伏期时间较第一阶段明显增加(P均<0.05)。HE染色观察到第一阶段有部分模型脊髓没有完全横断性损伤,第三阶段全部脊髓呈现完全横断性损伤。结论初学者经过耐心反复的训练,可通过学习曲线,于第三阶段,熟练掌握钳夹伤制作脊髓损伤模型。Objective To analyze the learning curve of establishing rat models of spinal cord injury by clip compression. Methods The data of the first 30 consecutive cases of rat models of spinal cord injury lay clip compression were collected for this retrospective study. The models were divided into three stages, and each group had 10 cases. Operation time, the volume of blood loss,the rate of spinal cord injury in the sham operation group,post-operative infection, Basso-Beattie-Breshahan （BBB） locomotor rating scale for spinal cord injury, motor evoked potentials N1 （MEP-N1） peak were analyzed. Pathological HE staining was also performed. Results The third stage of operation had obviously shorter operation time, less volume of blood loss, less spinal cord injury happening in the sham operation group,less post-operative infection, lower BBB scores and longer latencies of MEP-N1 peak, compared with the first stage, there were statistical differences （all P〈0.05）. HE staining presented that at the first stage some models had no completely transactional spinal cord injury, and at the third stage all models had completely transactional spinal cord injury. Conclusion Learners could grasp these skills by the learning curve with patient and repeated practice, at the third stage, any model suitable for experimental study of spinal cord injury by clip compression could be established successfully.

关 键 词：脊髓损伤 模型 大鼠 学习曲线 

分 类 号：R744[医药卫生—神经病学与精神病学]