厚朴酚抑制体外乳腺癌细胞株生物活性的实验研究  

Experimental Study on the Inhibition of Magnolol on Biological Activities of Human Breast Cancer Cell Lines in Vitro

作　　者：黄自明[1] 姜冉[1] 魏刚[1] 田亦文 卢文亮[1] 高丹[1] 陈波[1] 叶春梅[1] 薛明兴[1] 王蓉[1] 

机构地区：[1]湖北省妇幼保健院甲乳外科,湖北武汉430070

出　　处：《医学临床研究》2017年第6期1047-1050,共4页Journal of Clinical Research

基　　金：湖北省自然科技基金项目（2014CFB472）

摘　　要：【目的】探讨中药厚朴提取物厚朴酚（Magnolol，Mag）抑制不同乳腺癌细胞株生物活性的作用。【方法】选取不同乳腺癌高转移细胞株（MCF-7、MDA-MB-231），在培养液中分别加入不同浓度的Mag溶液，观察处理后细胞的凋亡、侵袭、转移和黏附能力，判断Mag对乳腺癌细胞株生物活性的影响。【结果]Mag可以抑制细胞的抗凋亡、侵袭、转移和黏附能力（P〈O．05），随着Mag浓度的增大，抑制生物活性的作用逐渐增强（P〈0．05）。【结论]Mag可以通过抑制不同受体状态乳腺癌细胞株的抗凋亡率、侵袭能力、黏附能力和迁移能力，从而抑制其生物活性，在乳腺癌的治疗及预防乳腺癌复发、转移中具有重要的临床应用价值。[Objective]To investigate the effects of Magnolia （Magnolol, Mag） that inhibited the biological ac- tivity of different breast cancer cell lines in vitro. [Methods] Human breast cancer high metastatic cell lines MCF- 7 and MDA MB-231 were used in the study. Different concentrations of Mag solution were added to the culture medium to observe the rate of apoptosis, invasion, migration and adhesion capacity. Then, the effects of Mag on biological activity of breast cancer cell line were evaluated. [ResultslData showed that Mag inhibited the rate of ap- optosis, invasion, metastasis and adhesion ability in two breast cancer cell lines （ P 〈0.05）, and that with the in- crease of Mag concentration, the inhibitory activity on the biological activity was increased （ P〈0.05）. [Conclu- sion]Mag can inhibit the biological activities of breast cancer cell lines by inhibiting the apoptotic rate, the ability of invasion, and the adhesion and migration of breast cancer cell lines. It has an important value in the treatment and prevention of breast cancer recurrence or metastasis in clinic.

关 键 词：厚朴酚/药理学 乳腺肿瘤/药物疗法 肿瘤细胞 培养的 肿瘤转移 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]