TRIM55在结直肠癌中的表达及其临床意义  

Expression and Clinical Significance of TRIM55 in Colorectal Cancer

作　　者：梁倩[1] 房静远[1] 张洁[2] 洪洁[1] 

机构地区：[1]上海交通大学医学院附属仁济医院消化科上海市消化疾病研究所,200001 [2]上海交通大学医学院附属仁济医院检验科

出　　处：《胃肠病学》2017年第6期341-345,共5页Chinese Journal of Gastroenterology

基　　金：临床专职科研队伍(20152512)资助

摘　　要：背景:TRIM55为TRIM蛋白家族成员,大部分TRIM蛋白含环指结构域,可发挥E3泛素连接酶的作用,与癌症的发生、发展密切相关。目的:探讨TRIM55在结直肠癌中的表达及其临床意义,探究可能的作用机制。方法:选取2014年10月—2015年12月上海交通大学医学院附属仁济医院的70例结直肠癌组织及其相应癌旁组织,以实时PCR法检测TRIM55表达。将TRIM55小干扰RNA(siRNA)转染人结直肠癌细胞株HCT116,以CCK-8法检测细胞增殖,蛋白质印迹法检测TRIM55、SOCS1蛋白表达。结果:49例结直肠癌组织中TRIM55表达高于癌旁组织,且TRIM55高表达与肿瘤分化程度(P=0.032)、AJCC分期(P=0.001)和淋巴结转移相关(P=0.001),而与患者的性别、年龄、肿瘤大小、浸润深度、远处转移和脉管侵犯无关(P>0.05)。TRIM55 siRNA可有效下调HCT116细胞中TRIM55 mRNA和蛋白表达(P<0.01),降低细胞增殖能力(P<0.01),并上调SOCS1蛋白表达(P<0.01)。结论:E3泛素连接酶TRIM55可能通过影响SOCS1的表达促进结直肠癌细胞增殖,进而促进结直肠癌的疾病进程,提示检测TRIM55表达有望为结直肠癌的诊断和治疗提供新思路。Background： TRIM55 is a member of TRIM family. Most TRIM proteins,which can be defined as E3 ubiquitin ligase because of the RING-finger domain,are closely related with the initiation and progression of cancer. Aims： To study the expression and clinical significance of TRIM55 in colorectal cancer,and explore the potential mechanism of TRIM55 in colorectal cancer. Methods： Seventy colorectal cancer tissues and corresponding paracancerous tissues taken from colorectal cancer patients from October 2014 to December 2015 at Shanghai Ren Ji Hospital were enrolled. Real-time PCR was performed to examine the expression of TRIM55. Human colorectal cancer cell line HCT116 was transfected with TRIM55 small interfering RNA（ siRNA）, cell proliferation was measured by CCK-8 assay, Western blotting was implemented to determine the protein expressions of TRIM55 and SOCS1. Results： The expression of TRIM55 was significantly increased in 49 colorectal cancer tissues than in corresponding paracancerous tissues. Increased expression of TRIM55 was closely correlated with tumor differentiation（ P = 0. 032）,AJCC staging（ P = 0. 001） and lymph node metastasis（ P = 0. 001）,but not related to gender,age,tumor size,invasion depth,distant metastasis and vascular invasion（ P〉 0. 05）. After transfection with TRIM55 siRNA,mRNA and protein expressions of TRIM55 were significantly decreased（ P〈0. 01）,proliferation of HCT116 cells was significantly decreased（ P〈0. 01）,and protein expression of SOCS1 was significantly increased（ P〈0. 01）. Conclusions： E3 ubiquitin ligase TRIM55 may promote the proliferation of colorectal cancer cells via influencing the expression of SOCS1,thus promoting the progression of colorectal cancer. This indicates that detection of TRIM55 expression may provide a new approach for diagnosis and therapy of colorectal cancer.

关 键 词：泛素化 TRIM55 结直肠肿瘤 RNA 小分子干扰 细胞增殖 细胞因子信号转导蛋白抑制因子 

分 类 号：R735.34[医药卫生—肿瘤]